PMID- 11916931
OWN - NLM
STAT- MEDLINE
DCOM- 20020510
LR  - 20220311
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 51
IP  - 4
DP  - 2002 Apr
TI  - Modulation of adipose tissue expression of murine matrix metalloproteinases and 
      their tissue inhibitors with obesity.
PG  - 1093-101
AB  - The potential role of the matrix metalloproteinase (MMP) system in the 
      pathophysiology of the adipose tissue was investigated in a mouse model of 
      nutritionally induced obesity. mRNA levels of 16 MMPs and 4 tissue inhibitors of 
      MMPs (TIMPs) were measured by semiquantitative RT-PCR in adipose tissue isolated 
      from mice maintained for 15 weeks on a standard or high-fat diet. In mice on 
      standard diet, with the exception of MMP-8, all MMP and TIMP transcripts were 
      detected in both gonadal and subcutaneous depots. In obese mice, the expression 
      of MMP-3, -11, -12, -13, and -14 and TIMP-1 mRNAs was upregulated, whereas that 
      of MMP-7, -9, -16, and -24 and TIMP-4 was downregulated. Most MMP and TIMP mRNAs 
      were expressed at higher levels in stromal-vascular cells than in mature 
      adipocytes. Analysis of adipose tissue by in situ fluorescent zymography revealed 
      MMP-dependent proteolytic activities, demonstrating the presence of active MMPs 
      in the intact tissue. In vitro conversion of adipogenic 3T3-F442A cells into 
      mature adipocytes was associated with substantial modulations of MMP and TIMP 
      expression. Moreover, this in vitro adipogenesis was reduced in the presence of a 
      synthetic MMP inhibitor. Thus, the adipose tissue expresses a large array of MMPs 
      and TIMPs, which modulate adipocyte differentiation.
FAU - Maquoi, Erik
AU  - Maquoi E
AD  - Center for Molecular and Vascular Biology, University of Leuven, Belgium.
FAU - Munaut, Carine
AU  - Munaut C
FAU - Colige, Alain
AU  - Colige A
FAU - Collen, Desire
AU  - Collen D
FAU - Lijnen, H Roger
AU  - Lijnen HR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/cytology/metabolism
MH  - Adipose Tissue/*metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - Diet
MH  - Enzyme Inhibitors/pharmacology
MH  - Epididymis
MH  - Gene Expression Regulation/*physiology
MH  - Lung/metabolism
MH  - Male
MH  - Matrix Metalloproteinases/*genetics
MH  - Mice
MH  - Obesity/*genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinases/*genetics
MH  - Transcription, Genetic
EDAT- 2002/03/28 10:00
MHDA- 2002/05/11 10:01
CRDT- 2002/03/28 10:00
PHST- 2002/03/28 10:00 [pubmed]
PHST- 2002/05/11 10:01 [medline]
PHST- 2002/03/28 10:00 [entrez]
AID - 10.2337/diabetes.51.4.1093 [doi]
PST - ppublish
SO  - Diabetes. 2002 Apr;51(4):1093-101. doi: 10.2337/diabetes.51.4.1093.