PMID- 11918087
OWN - NLM
STAT- MEDLINE
DCOM- 20020422
LR  - 20191025
IS  - 0262-0898 (Print)
IS  - 0262-0898 (Linking)
VI  - 19
IP  - 1
DP  - 2002
TI  - Monocyte chemoattractant protein-1 (MCP-1), secreted by bone marrow endothelial 
      cells, induces chemoattraction of 5T multiple myeloma cells.
PG  - 87-90
AB  - Homing of multiple myeloma (MM) cells to the bone marrow (BM) requires 
      transendothelial migration. In the present work we tested whether monocyte 
      chemoattractant protein-1 (MCP-1) and CCR2, the high affinity receptor for MCP-1, 
      are involved in this process. Murine 5T2 and 5T33MM cell lines were selected as 
      source of MM cells and STR4, 10 and 12 of BM endothelial cells (BMEC). RT-PCR 
      demonstrated transcripts for MCP-1 in BMEC and ELISA the presence of MCP-1 
      protein in culture medium. RNase protection assay showed mRNA expression for 
      CCR2, and FACS analysis the presence of CCR2 protein on the MM cells. EC 
      conditioned medium induced chemoattraction of MM cells, a phenomenon inhibited by 
      anti-MCP-1 antibodies. In conclusion, MM cells express CCR2 and are attracted by 
      MCP-1 secreted by BMEC. We suggest that local MCP-1 production by BMEC is one of 
      the mechanisms involved in homing of myeloma cells to the BM.
FAU - Vanderkerken, Karin
AU  - Vanderkerken K
AD  - Department of Hematology and Immunology, Free University Brussels (VUB), Belgium. 
      Karin.Vanderkerken@vub.ac.be
FAU - Vande Broek, Isabelle
AU  - Vande Broek I
FAU - Eizirik, Decio L
AU  - Eizirik DL
FAU - Van Valckenborgh, Els
AU  - Van Valckenborgh E
FAU - Asosingh, Kewal
AU  - Asosingh K
FAU - Van Riet, Ivan
AU  - Van Riet I
FAU - Van Camp, Ben
AU  - Van Camp B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Exp Metastasis
JT  - Clinical & experimental metastasis
JID - 8409970
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - Bone Marrow/*metabolism
MH  - Cell Line, Transformed
MH  - Chemokine CCL2/metabolism/*pharmacology
MH  - Chemotaxis/*drug effects
MH  - Endothelium/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Multiple Myeloma/genetics/*pathology
MH  - Neoplasm Proteins/drug effects/genetics/physiology
MH  - RNA, Messenger/analysis
MH  - RNA, Neoplasm/analysis
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/drug effects/genetics/physiology
EDAT- 2002/03/29 10:00
MHDA- 2002/04/23 10:01
CRDT- 2002/03/29 10:00
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/04/23 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - 10.1023/a:1013891205989 [doi]
PST - ppublish
SO  - Clin Exp Metastasis. 2002;19(1):87-90. doi: 10.1023/a:1013891205989.