PMID- 11918774
OWN - NLM
STAT- MEDLINE
DCOM- 20020521
LR  - 20190916
IS  - 0962-1083 (Print)
IS  - 0962-1083 (Linking)
VI  - 11
IP  - 3
DP  - 2002 Mar
TI  - Insertion polymorphism of retrotransposable elements in populations of the 
      insular, endemic species Drosophila madeirensis.
PG  - 347-54
AB  - The insertion site numbers of the retrotransposable elements (TE) 412, gypsy and 
      bilbo were determined in individuals of five distinct natural populations of the 
      endemic species Drosophila madeirensis from the island of Madeira. The TE 
      distributions were compared to those of the paleartic, widespread and 
      phylogenetically closely related species, D. subobscura. In situ hybridization 
      and Southern blots showed that in D. madeirensis the number of insertion sites 
      ranged between 10 and 15, three and six, and 35 and 42 for elements 412, gypsy 
      and bilbo, respectively. The corresponding values for D. subobscura were similar. 
      Two of these elements, 412 and gypsy, had very few insertions in the 
      heterochromatin, unlike bilbo, which displayed a high heterochromatic insertion 
      number. The Southern band polymorphism was very high, leading to 
      within-population variation of 97.2%, whatever the population and the TE 
      concerned. Using the polymorphic TE insertion sites as markers to analyse 
      population structure by AMOVA, adapted for RAPD (Randomly Amplified Polymorphic 
      DNA) data, we found small but significant genetic differences between the 
      populations on Madeira. This slight differentiation, coupled with similar copy 
      numbers for each TE between populations, suggests that the D. madeirensis species 
      consists of a single, only slightly subdivided population. These data also show 
      that insular populations and endemic species of Drosophila can have as many 
      copies of TEs as more widespread species.
FAU - Lepetit, D
AU  - Lepetit D
AD  - UMR CNRS. 5558: Biometrie et Biologie Evolutive, Universite Lyon 1, 69622 
      Villeurbanne Cedex, France.
FAU - Brehm, A
AU  - Brehm A
FAU - Fouillet, P
AU  - Fouillet P
FAU - Biemont, C
AU  - Biemont C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Ecol
JT  - Molecular ecology
JID - 9214478
RN  - 0 (Heterochromatin)
RN  - 0 (Retroelements)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - DNA/genetics/metabolism
MH  - Drosophila/*genetics
MH  - Heterochromatin/genetics/metabolism
MH  - In Situ Hybridization
MH  - *Polymorphism, Genetic
MH  - Portugal
MH  - Retroelements/*genetics
EDAT- 2002/03/29 10:00
MHDA- 2002/05/22 10:01
CRDT- 2002/03/29 10:00
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/05/22 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - 1470 [pii]
AID - 10.1046/j.1365-294x.2002.01470.x [doi]
PST - ppublish
SO  - Mol Ecol. 2002 Mar;11(3):347-54. doi: 10.1046/j.1365-294x.2002.01470.x.