PMID- 11918969
OWN - NLM
STAT- MEDLINE
DCOM- 20020813
LR  - 20190901
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 41
IP  - 1
DP  - 2002 Jul
TI  - Effects of different oxidizing agents on neutral amino acid transport systems in 
      isolated bovine brain microvessels.
PG  - 29-36
AB  - Using isolated bovine brain microvessels as an in vitro model of the blood-brain 
      barrier (BBB) we have evaluated the role of free radical generating solutions on 
      some amino acid transport systems operating on the endothelial cell membrane. 
      Fe(2+)/ascorbate, phenylhydrazine and CuSO(4) did not affect any of the transport 
      system tested, while exposure of bovine brain microvessels to 
      tert-butylhydroperoxide (t-BHP) caused a reduced capacity to take up small 
      neutral amino acids via the Na(+)-dependent A-system. The presence of glucose 
      during t-BHP treatment did not prevent this inhibition, which was partially 
      counteracted when the isolated microvessels were incubated with 5mM inosine 
      before the oxidative stress. Incubation of the isolated capillaries with 5mM 
      dithiothreitol, after exposure to t-BHP, resulted in a 50% recovery of the 
      alpha-methylaminoisobutyrate (MeAIB) uptake by the A-system. Treatment with 
      t-BHP, which had no effect on the L-system of neutral amino acid transport, 
      caused a significant decrease of the intracellular levels of ATP, of glutathione 
      (GSH), and of gamma-glutamyltranspeptidase (GGT) activity, while no significant 
      modification of hexokinase (HK) or of alkaline phosphatase (ALKP) activities were 
      observed. Oxidative damage of the BBB appears therefore to impair essentially the 
      metabolic pathways which ensure the energy requirement for the endothelial cells, 
      thus inhibiting the energy-dependent amino acid transport system "A".
FAU - Cardelli, Patrizia
AU  - Cardelli P
AD  - Department of Cellular Biotechnology and Hematology, University of Rome, La 
      Sapienza, Italy.
FAU - Scarpa, Sigfrido
AU  - Scarpa S
FAU - Ceci, Fabrizio
AU  - Ceci F
FAU - Lucarelli, Marco
AU  - Lucarelli M
FAU - Tabacco, Fabio
AU  - Tabacco F
FAU - Ferraguti, Giampiero
AU  - Ferraguti G
FAU - Brisdelli, Fabrizia
AU  - Brisdelli F
FAU - Strom, Roberto
AU  - Strom R
FAU - Bozzi, Argante
AU  - Bozzi A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Amino Acid Transport Systems, Neutral)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Amino Acid Transport Systems, Neutral/*drug effects
MH  - Animals
MH  - Brain/*blood supply
MH  - Cattle
MH  - Cerebrovascular Circulation/*drug effects
MH  - Glutathione/metabolism
MH  - In Vitro Techniques
MH  - Oxidation-Reduction
EDAT- 2002/03/29 10:00
MHDA- 2002/08/14 10:01
CRDT- 2002/03/29 10:00
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/08/14 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - S0197018601001395 [pii]
AID - 10.1016/s0197-0186(01)00139-5 [doi]
PST - ppublish
SO  - Neurochem Int. 2002 Jul;41(1):29-36. doi: 10.1016/s0197-0186(01)00139-5.