PMID- 11919710
OWN - NLM
STAT- MEDLINE
DCOM- 20020510
LR  - 20151119
IS  - 1617-4615 (Print)
IS  - 1617-4623 (Linking)
VI  - 267
IP  - 1
DP  - 2002 Mar
TI  - Tetrad-FISH analysis reveals recombination suppression by interstitial 
      heterochromatin sequences in rye (Secale cereale).
PG  - 10-5
AB  - Tetrad analysis is a genetic method that can locate genes and centromeres on a 
      linkage map with a high degree of precision. Despite its effectiveness and 
      accuracy, application of this method is generally limited to fungi, algae and 
      mosses. Here we demonstrate a new method of tetrad analysis that is applicable to 
      other organisms. This combines tetrad analysis with fluorescence in situ 
      hybridization (FISH), and is thus referred to as tetrad-FISH analysis. We 
      demonstrate the effectiveness of this method using tetrads of rye, Secale 
      cereale. The rye strain JNK contains interstitial heterochromatin in a region of 
      Chromosome 2R. We have previously cloned the tandemly repeated sequence forming 
      this heterochromatin in the plasmid pScJNK. We performed FISH using pScJNK as the 
      probe on tetrads obtained from heterozygotes for the heterochromatin region. The 
      frequency of tetrads demonstrating positive signals in two cells that are 
      diagonally opposite one another must correspond to the frequency of recombination 
      in the interval between the heterochromatin and the centromere. Comparison 
      between the results of tetrad-FISH analysis and linkage maps based on RFLP 
      markers clearly indicated that heterochromatin strongly suppresses recombination 
      of whole chromosomal regions. We discuss the effectiveness of tetrad-FISH 
      analysis, particularly for the localization of functional centromeres in linkage 
      maps.
FAU - Kagawa, N
AU  - Kagawa N
AD  - Kihara Institute for Biological Research and Graduate School of Integrated 
      Sciences, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama 
      244-0813, Japan.
FAU - Nagaki, K
AU  - Nagaki K
FAU - Tsujimoto, H
AU  - Tsujimoto H
LA  - eng
PT  - Journal Article
DEP - 20020202
PL  - Germany
TA  - Mol Genet Genomics
JT  - Molecular genetics and genomics : MGG
JID - 101093320
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Genetic Linkage
MH  - Heterochromatin/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Recombination, Genetic
MH  - Secale/*genetics
EDAT- 2002/03/29 10:00
MHDA- 2002/05/11 10:01
CRDT- 2002/03/29 10:00
PHST- 2001/11/19 00:00 [received]
PHST- 2001/12/13 00:00 [accepted]
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/05/11 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - 10.1007/s00438-001-0634-5 [doi]
PST - ppublish
SO  - Mol Genet Genomics. 2002 Mar;267(1):10-5. doi: 10.1007/s00438-001-0634-5. Epub 
      2002 Feb 2.