PMID- 11920190
OWN - NLM
STAT- MEDLINE
DCOM- 20020508
LR  - 20191105
IS  - 1466-4860 (Print)
IS  - 1466-4860 (Linking)
VI  - 1
IP  - 3
DP  - 2000
TI  - Role of plasminogen activators and urokinase receptor in platelet kinetics.
PG  - 199-205
AB  - INTRODUCTION: Plasminogen activators (PA) and plasmin are known to affect 
      platelets but little is known of their role in platelet kinetics. We took 
      advantage of genetically deficient mice to explore the role of urokinase (uPA) 
      and tissue type (tPA) PAs, as well as the uPA receptor (uPAR, CD87) in platelet 
      kinetics. MATERIALS AND METHODS: Platelet shape and number were investigated by 
      flow cytometry. Platelet kinetics was investigated by the in vivo biotinylation 
      and FACS analysis. Platelet production was investigated by counting 
      megakaryocytes in bone marrow. RESULTS: Platelets counts were within the same 
      range in wild type (+/+), uPA, tPA and uPAR-deficient mice. Platelet survival was 
      similar in +/+, uPA-/-, tPA-/- but markedly reduced in uPAR-/- mice. The number 
      of megakaryocytes in bone marrow and spleen was increased 2-3-fold in uPAR-/- 
      compared to +/+ mice. TGF-beta mRNA level within the bone marrow was also 
      significantly increased in uPAR-/- mice. Consistent with an increased platelet 
      production, platelets from uPAR-/- mice had a higher RNA content, as seen by 
      Propidium Iodide (PI) labeling and FACS analysis. Since uPAR is detectable in 
      both hemopoietic and non-hemopoietic cells, radiation chimera were prepared. 
      Investigation of platelet kinetics in chimera showed that platelet survival is 
      reduced with a deficit in either bone marrow-derived, or non-hemopoietic, host 
      cells. CONCLUSION: These results demonstrate that uPAR, but not uPA or tPA, is 
      essential for maintaining normal platelet survival. In addition, uPAR-/- mice 
      maintain normal platelet numbers through increased production.
FAU - Piguet, P F
AU  - Piguet PF
AD  - Department of Pathology, University of Geneva, CH 1211, Switzerland. 
      pierre.piguet@medecine.unige.ch
FAU - Vesin, C
AU  - Vesin C
FAU - Da Laperousaz, C
AU  - Da Laperousaz C
FAU - Rochat, A
AU  - Rochat A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hematol J
JT  - The hematology journal : the official journal of the European Haematology 
      Association
JID - 100965523
RN  - 0 (Plaur protein, mouse)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - EC 3.4.21.- (Plasminogen Activators)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.21.7 (Fibrinolysin)
SB  - IM
MH  - Animals
MH  - Blood Platelets/*physiology
MH  - Fibrinolysin/metabolism
MH  - Kinetics
MH  - Megakaryocytes/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Plasminogen Activators/*blood
MH  - Receptors, Cell Surface/*blood/deficiency/genetics
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Tissue Plasminogen Activator/blood
EDAT- 2002/03/29 10:00
MHDA- 2002/05/09 10:01
CRDT- 2002/03/29 10:00
PHST- 1999/10/19 00:00 [received]
PHST- 2000/03/10 00:00 [accepted]
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/05/09 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - 10.1038/sj.thj.6200029 [doi]
PST - ppublish
SO  - Hematol J. 2000;1(3):199-205. doi: 10.1038/sj.thj.6200029.