PMID- 11920386
OWN - NLM
STAT- MEDLINE
DCOM- 20020702
LR  - 20190116
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 266
IP  - 4
DP  - 2002 Apr 1
TI  - Cell kinetic study of the endometrium by nonisotopic in situ hybridization for 
      histone H3 messenger RNA and immunohistochemistry for Ki-67 and for estrogen and 
      progesterone receptors.
PG  - 234-40
AB  - We investigated the cell kinetics of the endometrium in hysterectomy specimens 
      taken for leiomyoma from 22 women with regular ovulatory menstrual cycles. 
      Formalin-fixed, paraffin-embedded tissue sections were examined for proliferating 
      activity using histone H3 messenger RNA in situ hybridization (H3 mRNA-ISH) and 
      immunostaining for the Ki-67 antigen. The relationship of the proliferative 
      activity of endometrial cells to the immunohistochemical expression of the 
      estrogen receptor (ER) and the progesterone receptor (PR) was also examined. 
      During the menstrual cycle, H3 mRNA expression was observed in both the 
      epithelial cells and the stromal cells of the endometrium. In the functional 
      layer, the labeling indices for H3 mRNA (H3 mRNA-LIs) in the epithelial cells 
      peaked in the late proliferative phase, decreased sharply in the early secretory 
      phase, and remained unchanged thereafter. On the other hand, H3 mRNA-LIs of 
      stromal cells displayed two peaks: one in the midproliferative phase and the 
      other in the late secretory phase, the former peak being the greater. In the 
      basal layer, epithelial cells and stromal cells showed low H3 mRNA-LIs and no 
      significant variation throughout the menstrual cycle. The H3 mRNA-LIs correlated 
      well with the Ki-67-LIs and were lower than the corresponding Ki-67-LIs. The 
      regression coefficient (H3 mRNA-LIs against the Ki-67-LIs) was 0.33 for 
      epithelial cells and 0.49 for stromal cells, suggesting that the cell cycle time 
      was longer for epithelial cells than for stromal cells. The proliferative 
      activity of endometrial cells showed close relationships with the expressions of 
      ER and PR in the endometrium. When used in combination with other proliferative 
      markers in paraffin-embedded tissue sections, H3 mRNA-ISH could open broader 
      perspectives on the cell kinetics of the endometrium.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Hayama, Masayoshi
AU  - Hayama M
AD  - Department of Laboratory Medicine, Shinshu University, Matsumoto, Nagano, Japan.
FAU - Ota, Hiroyoshi
AU  - Ota H
FAU - Toki, Toshihiko
AU  - Toki T
FAU - Ishii, Keiko
AU  - Ishii K
FAU - Honda, Takayuki
AU  - Honda T
FAU - Momose, Masanobu
AU  - Momose M
FAU - Nakata, Ritsuko
AU  - Nakata R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 0 (Histones)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
SB  - IM
MH  - Adult
MH  - Cell Division/physiology
MH  - Endometrium/cytology/*metabolism
MH  - Female
MH  - Histones/*biosynthesis/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Ki-67 Antigen/metabolism
MH  - Kinetics
MH  - Menstrual Cycle/*physiology
MH  - Middle Aged
MH  - RNA, Messenger/*biosynthesis
MH  - Receptors, Estrogen/*metabolism
MH  - Receptors, Progesterone/*metabolism
EDAT- 2002/03/29 10:00
MHDA- 2002/07/03 10:01
CRDT- 2002/03/29 10:00
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/07/03 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - 10.1002/ar.10064 [pii]
AID - 10.1002/ar.10064 [doi]
PST - ppublish
SO  - Anat Rec. 2002 Apr 1;266(4):234-40. doi: 10.1002/ar.10064.