PMID- 11920816
OWN - NLM
STAT- MEDLINE
DCOM- 20020531
LR  - 20190901
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 67
IP  - 1
DP  - 2002 May
TI  - Cytokine gene expression and protein production in peripheral blood mononuclear 
      cells of children with acute dengue virus infections.
PG  - 41-6
AB  - Plasma leakage in dengue hemorrhagic fever (DHF) is associated with elevated 
      plasma levels of cytokines. To define further the contribution of immune 
      activation to DHF and the source of cytokines, we analyzed the production of 
      cytokines in peripheral blood mononuclear cells (PBMC) obtained from children 
      with dengue, using RT-PCR and immunostaining. Tumor necrosis factor-alpha 
      (TNF-alpha) and TNF-beta expression was detected in all samples by PCR and in < 
      50% of samples by immunostaining. Interferon-gamma (IFN-gamma) expression was 
      detected in < 50% of samples by either method. Interleukin-2 (IL-2) and IL-4 
      expression was detected in a few samples by immunostaining but was not detectable 
      by PCR. We found greater expression of TNF-alpha and IL-4 in DHF than in dengue 
      fever or other (non-dengue) febrile illnesses. These results support the model of 
      immunopathogenesis of DHF. However, low levels of cytokine expression in PBMC 
      suggest that cellular activation in tissues may contribute to high serum cytokine 
      levels in DHF.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Gagnon, Susan J
AU  - Gagnon SJ
AD  - Center for Infectious Disease and Vaccine Research, University of Massachusetts 
      Medical School, Worcester, Massachusetts 01655, USA.
FAU - Mori, Masuko
AU  - Mori M
FAU - Kurane, Ichiro
AU  - Kurane I
FAU - Green, Sharone
AU  - Green S
FAU - Vaughn, David W
AU  - Vaughn DW
FAU - Kalayanarooj, Siripen
AU  - Kalayanarooj S
FAU - Suntayakorn, Saroj
AU  - Suntayakorn S
FAU - Ennis, Francis A
AU  - Ennis FA
FAU - Rothman, Alan L
AU  - Rothman AL
LA  - eng
GR  - P01 AI34533/AI/NIAID NIH HHS/United States
GR  - R01 AI30624/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-2)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/*biosynthesis/genetics
MH  - Female
MH  - Fever/immunology
MH  - *Gene Expression
MH  - Humans
MH  - Infant
MH  - Interleukin-2/biosynthesis/genetics
MH  - Interleukin-4/biosynthesis/genetics
MH  - Leukocytes, Mononuclear/immunology
MH  - Lymphotoxin-alpha/biosynthesis/genetics
MH  - Male
MH  - Severe Dengue/blood/*immunology
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 2002/03/29 10:00
MHDA- 2002/06/01 10:01
CRDT- 2002/03/29 10:00
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/06/01 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - 10.1002/jmv.2190 [pii]
AID - 10.1002/jmv.2190 [doi]
PST - ppublish
SO  - J Med Virol. 2002 May;67(1):41-6. doi: 10.1002/jmv.2190.