PMID- 11921276
OWN - NLM
STAT- MEDLINE
DCOM- 20020419
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 34
IP  - 1
DP  - 2002 May
TI  - Comprehensive karyotyping of the HT-29 colon adenocarcinoma cell line.
PG  - 1-8
AB  - The tumor cell line HT-29 was derived from a primary adenocarcinoma of the 
      rectosigmoid colon. HT-29 is hypertriploid (3n+) and has accumulated numerous 
      chromosomal structural aberrations. To identify material involved in chromosome 
      rearrangements, we performed a comprehensive cytogenetic analysis using 
      G-banding, spectral karyotyping (SKY), and fluorescence in situ hybridization 
      (FISH). The combination of molecular cytogenetic techniques enabled us to define 
      the first comprehensive karyotype for HT-29. Seventeen marker chromosomes were 
      found in 75-100% of metaphase cells, generally in a single copy per cell. We 
      confirmed the composition of eight previously described markers, refined the 
      classification of seven others, and identified two novel marker chromosomes. 
      Notable aberrations included a reciprocal translocation between chromosomes 6 and 
      14 and an unusual, large derivative chromosome 8 composed entirely of 8q 
      material. The telomere status, evaluated by FISH, revealed telomeric signals at 
      the termini of all chromosomes. No interstitial telomeric sequences were observed 
      in any cell. Although numerous chromosomal aberrations are present in HT-29, the 
      cell line appears to have retained a high level of genomic stability during 
      passage in culture since undergoing transformation. The excellent resolving power 
      of SKY, coupled with additional information obtained from molecular cytogenetic 
      analyses, will improve our ability to identify genetic lesions characteristic of 
      cancer.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Kawai, Kanji
AU  - Kawai K
AD  - UCSD Cancer Center and Department of Pathology, University of California San 
      Diego, La Jolla, California 92093-0912, USA.
FAU - Viars, Carrie
AU  - Viars C
FAU - Arden, Karen
AU  - Arden K
FAU - Tarin, David
AU  - Tarin D
FAU - Urquidi, Virginia
AU  - Urquidi V
FAU - Goodison, Steve
AU  - Goodison S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Genetic Markers)
SB  - IM
CIN - Genes Chromosomes Cancer. 2003 Feb;36(2):207-10. PMID: 12508250
MH  - Adult
MH  - Chromosome Aberrations
MH  - Female
MH  - Genetic Markers/genetics
MH  - HT29 Cells/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Telomere/genetics
EDAT- 2002/03/29 10:00
MHDA- 2002/04/20 10:01
CRDT- 2002/03/29 10:00
PHST- 2002/03/29 10:00 [pubmed]
PHST- 2002/04/20 10:01 [medline]
PHST- 2002/03/29 10:00 [entrez]
AID - 10.1002/gcc.10003 [pii]
AID - 10.1002/gcc.10003 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2002 May;34(1):1-8. doi: 10.1002/gcc.10003.