PMID- 11923382
OWN - NLM
STAT- MEDLINE
DCOM- 20020716
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 4
DP  - 2002 Apr
TI  - Novel IS6110 insertion sites in the direct repeat locus of Mycobacterium 
      tuberculosis clinical strains from the St. Petersburg area of Russia and 
      evolutionary and epidemiological considerations.
PG  - 1504-7
AB  - A modification of spoligotyping with primers derived from the direct repeat (DR) 
      and IS6110 sequences was used to identify IS6110 insertions in the DR locus of 
      Mycobacterium tuberculosis clinical strains from the St. Petersburg area of 
      Russia. Novel IS6110 insertions were identified: (i) in two epidemiologically 
      unlinked Beijing family strains, an asymmetrical direct insertion in DR37; (ii) 
      in a non-Beijing strain, an asymmetrical insertion in the opposite orientation in 
      DR38; (iii) in another non-Beijing strain, a direct insertion in DR38 and one in 
      the opposite orientation in DR14 (DR numbering is according to standard 
      spoligotyping). Our results strengthen an observation that the DR locus structure 
      is extremely conserved in the Beijing genotype. Asymmetrical insertions prevented 
      detection of the adjacent spacer by standard spoligotyping. This, therefore, 
      should be taken into consideration when similar spoligoprofiles that differ only 
      in signals 37 and 38 are interpreted.
FAU - Mokrousov, Igor
AU  - Mokrousov I
AD  - Laboratory of Molecular Microbiology, Pasteur Institute, St. Petersburg 197101, 
      Russia. miv@IM4520.spb.edu
FAU - Narvskaya, Olga
AU  - Narvskaya O
FAU - Limeschenko, Elena
AU  - Limeschenko E
FAU - Otten, Tatiana
AU  - Otten T
FAU - Vyshnevskiy, Boris
AU  - Vyshnevskiy B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - DNA Transposable Elements/*genetics
MH  - DNA, Bacterial/genetics
MH  - *Evolution, Molecular
MH  - Humans
MH  - Mycobacterium tuberculosis/*classification/genetics
MH  - Oligodeoxyribonucleotides/genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Russia/epidemiology
MH  - Tuberculosis, Pulmonary/*epidemiology/microbiology
PMC - PMC140396
EDAT- 2002/03/30 10:00
MHDA- 2002/07/18 10:01
PMCR- 2002/04/01
CRDT- 2002/03/30 10:00
PHST- 2002/03/30 10:00 [pubmed]
PHST- 2002/07/18 10:01 [medline]
PHST- 2002/03/30 10:00 [entrez]
PHST- 2002/04/01 00:00 [pmc-release]
AID - 1422 [pii]
AID - 10.1128/JCM.40.4.1504-1507.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 Apr;40(4):1504-7. doi: 10.1128/JCM.40.4.1504-1507.2002.