PMID- 11923430
OWN - NLM
STAT- MEDLINE
DCOM- 20020408
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 22
IP  - 7
DP  - 2002 Apr 1
TI  - Estrogen regulates the development of brain-derived neurotrophic factor mRNA and 
      protein in the rat hippocampus.
PG  - 2650-9
AB  - During development, estrogen has a variety of effects on morphological and 
      electrophysiological properties of hippocampal neurons. Brain-derived 
      neurotrophic factor (BDNF) also plays an important role in the survival and 
      differentiation of neurons during development. We examined the effects of 
      gonadectomy with and without estrogen replacement on the mRNA and protein of BDNF 
      and its receptor, trkB, during early postnatal development of the rat 
      hippocampus. We used immunocytochemistry to demonstrate that estrogen receptor 
      alpha (ERalpha) and BDNF were localized to the same cells within the developing 
      hippocampus. BDNF and ERalpha were colocalized in pyramidal cells of the CA3 
      subregion and to a lesser extent in CA1. To determine whether BDNF mRNA was 
      regulated by estrogen during development, we gonadectomized male rat pups at 
      postnatal day 0 (P0) and examined mRNA and protein levels from P0 to P25 using 
      real-time reverse transcription-PCR and Western blot analysis. After gonadectomy, 
      BDNF mRNA levels are significantly reduced on P7, but after treatment of 
      gonadectomized animals with estradiol benzoate on P0, levels at all ages were 
      similar to those in intact animals. BDNF mRNA changes after gonadectomy are 
      accompanied by an increase in the levels of BDNF protein, which were reduced by 
      estrogen treatment at P0. We also examined the effect of postnatal estrogen 
      treatment on trkB. There were no significant changes in trkB mRNA or protein in 
      gonadectomized or estrogen-replaced animals. These results suggest that a direct 
      interaction may exist between ERalpha and BDNF to alter hippocampal physiology 
      during development in the rat.
FAU - Solum, Derek T
AU  - Solum DT
AD  - Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, 
      Colorado 80523, USA.
FAU - Handa, Robert J
AU  - Handa RJ
LA  - eng
GR  - R01 AA012693/AA/NIAAA NIH HHS/United States
GR  - AA12693/AA/NIAAA NIH HHS/United States
GR  - F31 MH12292/MH/NIMH NIH HHS/United States
GR  - R01 NS039951/NS/NINDS NIH HHS/United States
GR  - F31 MH012292/MH/NIMH NIH HHS/United States
GR  - NS39951/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 1S4CJB5ZGN (estradiol 3-benzoate)
RN  - 4TI98Z838E (Estradiol)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Estradiol/*analogs & derivatives/pharmacology
MH  - Estrogen Receptor alpha
MH  - Estrogen Receptor beta
MH  - Estrogens/*metabolism/pharmacology
MH  - Female
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Hippocampus/drug effects/*growth & development/*metabolism
MH  - Immunohistochemistry
MH  - Interneurons/cytology/metabolism
MH  - Male
MH  - Orchiectomy
MH  - Ovariectomy
MH  - Pyramidal Cells/cytology/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/genetics/metabolism
MH  - Receptors, Estrogen/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - gamma-Aminobutyric Acid/metabolism
PMC - PMC6758321
EDAT- 2002/03/30 10:00
MHDA- 2002/04/12 10:01
PMCR- 2002/10/01
CRDT- 2002/03/30 10:00
PHST- 2002/03/30 10:00 [pubmed]
PHST- 2002/04/12 10:01 [medline]
PHST- 2002/03/30 10:00 [entrez]
PHST- 2002/10/01 00:00 [pmc-release]
AID - 22/7/2650 [pii]
AID - 6251 [pii]
AID - 10.1523/JNEUROSCI.22-07-02650.2002 [doi]
PST - ppublish
SO  - J Neurosci. 2002 Apr 1;22(7):2650-9. doi: 10.1523/JNEUROSCI.22-07-02650.2002.