PMID- 11925458
OWN - NLM
STAT- MEDLINE
DCOM- 20020426
LR  - 20180330
IS  - 0022-3166 (Print)
IS  - 0022-3166 (Linking)
VI  - 132
IP  - 4
DP  - 2002 Apr
TI  - Short-term zinc deficiency inhibits chondrocyte proliferation and induces cell 
      apoptosis in the epiphyseal growth plate of young chickens.
PG  - 665-73
AB  - The purpose of this study was to investigate the effect of zinc deficiency on 
      chondrocyte proliferation, differentiation and apoptosis in the epiphyseal growth 
      plate of juvenile chickens. Newly hatched broiler chickens were fed either a low 
      zinc (10 mg/kg diet) or a zinc-adequate (68 mg/kg diet) soy protein-based 
      purified diet. Cell proliferation in the growth plate was evaluated with 
      bromodeoxyuridine (BrdU) labeling. Apoptosis was assessed using the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method. 
      Chondrocyte differentiation was evaluated with immunostaining of osteonectin as a 
      marker of maturation. As early as d 3 of feeding, zinc deficiency significantly 
      inhibited chondrocyte proliferation, promoted cell differentiation and induced 
      cell apoptosis in the growth plate. These effects were manifested primarily in 
      areas remote from the blood supply. Immunostaining for local growth factors such 
      as insulin-like growth factor-1 (IGF-1), parathyroid hormone-related protein 
      (PTHrP) and fibroblast growth factor-2 (FGF-2) did not reveal any differences 
      between growth plates of zinc-deficient and zinc-adequate chickens after 3 d of 
      feeding. By d 7, severe growth plate lesions characterized by reduced cellularity 
      and abnormally shaped cells were formed in areas remote from blood vessels. 
      Immunoreactive IGF-1, PTHrP and FGF-2 were all greatly reduced in the lesion. 
      However, the growth rate and food intake of zinc-deficient chickens were not 
      different from those of the controls during the 7-d experiment. Therefore, a 
      direct effect of zinc deficiency on proliferation, differentiation, and apoptosis 
      of growth plate chondrocytes was indicated.
FAU - Wang, Xibin
AU  - Wang X
AD  - Department of Poultry Science, The Pennsylvania State University, University 
      Park, PA 16802-3501, USA.
FAU - Fosmire, Gary J
AU  - Fosmire GJ
FAU - Gay, Carol V
AU  - Gay CV
FAU - Leach, Roland M Jr
AU  - Leach RM Jr
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Antimetabolites)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antimetabolites/metabolism
MH  - Apoptosis/*physiology
MH  - Bromodeoxyuridine/metabolism
MH  - Chickens
MH  - Chondrocytes/*metabolism
MH  - Diet
MH  - Growth Plate/metabolism/*ultrastructure
MH  - Rats
MH  - Zinc/*deficiency
EDAT- 2002/04/02 10:00
MHDA- 2002/04/27 10:01
CRDT- 2002/04/02 10:00
PHST- 2002/04/02 10:00 [pubmed]
PHST- 2002/04/27 10:01 [medline]
PHST- 2002/04/02 10:00 [entrez]
AID - 10.1093/jn/132.4.665 [doi]
PST - ppublish
SO  - J Nutr. 2002 Apr;132(4):665-73. doi: 10.1093/jn/132.4.665.