PMID- 11927659
OWN - NLM
STAT- MEDLINE
DCOM- 20020502
LR  - 20081121
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 71
IP  - 4
DP  - 2002 Apr
TI  - Cytokine-mediated regulation of CXCR4 expression in human neutrophils.
PG  - 711-7
AB  - Several lines of evidence have suggested that a CXC chemokine receptor 4 
      (CXCR4)/stromal cell-derived factor-1 [SDF-1; CXC chemokine ligand 12 (CXCL12)] 
      pair is involved in baseline trafficking of leukocytes into extravascular tissues 
      and that modulation of surface CXCR4 expression may represent an alternative 
      mechanism for control of cell-specific biological responses to SDF-1/CXCL12. We 
      explored the regulation of CXCR4 expression by cytokines in polymorphonuclear 
      neutrophils (PMNs). No significant surface expression of CXCR4 in freshly 
      isolated PMNs was detected, but expression became apparent gradually during 
      incubation. SDF-1alpha/CXCL12 initiated Ca2+ mobilization and migratory responses 
      in 20 h cultured PMNs. The surface CXCR4 expression was suppressed most potently 
      by interferon-gamma (IFN-gamma). IFN-alpha, granulocyte-macrophage 
      colony-stimulating factor (GM-CSF), and G-CSF also inhibited spontaneous CXCR4 
      expression. Real-time, quantitative PCR experiments revealed that a spontaneous 
      increase and an IFN-gamma-mediated decrease in surface CXCR4 paralleled changes 
      in the CXCR4 mRNA level. These results on PMNs support the argument that the 
      SDF-1 (CXCL12)/CXCR4 system is regulated by cell type-specific mechanisms.
FAU - Nagase, Hiroyuki
AU  - Nagase H
AD  - Department of Respiratory Medicine, University of Tokyo Graduate School of 
      Medicine, Japan.
FAU - Miyamasu, Misato
AU  - Miyamasu M
FAU - Yamaguchi, Masao
AU  - Yamaguchi M
FAU - Imanishi, Masako
AU  - Imanishi M
FAU - Tsuno, Nelson H
AU  - Tsuno NH
FAU - Matsushima, Kouji
AU  - Matsushima K
FAU - Yamamoto, Kazuhiko
AU  - Yamamoto K
FAU - Morita, Yutaka
AU  - Morita Y
FAU - Hirai, Koichi
AU  - Hirai K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CXCR4)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cytokines/*pharmacology
MH  - Granulocyte Colony-Stimulating Factor/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Neutrophils/*metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, CXCR4/*biosynthesis/genetics
EDAT- 2002/04/03 10:00
MHDA- 2002/05/03 10:01
CRDT- 2002/04/03 10:00
PHST- 2002/04/03 10:00 [pubmed]
PHST- 2002/05/03 10:01 [medline]
PHST- 2002/04/03 10:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2002 Apr;71(4):711-7.