PMID- 11928988
OWN - NLM
STAT- MEDLINE
DCOM- 20030630
LR  - 20191025
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 24
IP  - 1
DP  - 2002
TI  - Varicella-zoster virus (VZV) mediates a delayed host shutoff independent of open 
      reading frame (ORF) 17 expression.
PG  - 49-56
AB  - Varicella-zoster virus (VZV) open reading frame 17 (ORF 17) is the gene 
      corresponding to Herpes simplex-virus (HSV) UL41. The UL41 gene encodes the 
      virion host shutoff factor (vhs), a RNase that has been the object of detailed 
      studies. In contrast to HSV, knowledge about VZV mediated shutoff effects and the 
      role of ORF 17 is poor. We investigated the ORF 17 expression in infected cells 
      and analyzed shutoff effects. ORF 17 expression could not be proven in infected 
      human fibroblast cell lines and melanoma (MeWo) cells. Only after induction by 
      Phorbol 12-myristate 13-acetate an ORF 17 expression became detectable in MeWo 
      cells. Nevertheless, using stable expressed GAPDH mRNA as a marker for mRNA 
      degradation, a VZV mediated shutoff, independent of ORF 17 expression, became 
      measurable. Transfection experiments demonstrated that transient ORF 17 
      expression did not decrease the cellular GAPDH mRNA level. We examined whether 
      the VZV shutoff factor is a tegument protein causing an early shutoff or whether 
      it needs to be expressed (delayed shutoff). The GAPDH mRNA level in Actinomycin D 
      pretreated and infected MeWo cells did not decrease even faster than the 
      theoretical decay rate based on a half-life of 24 h. These findings lead to the 
      conclusion that the VZV shutoff factor is not a mature protein localized in the 
      virion and that VZV causes a delayed virion host shutoff effect.
FAU - Waterboer, Tim
AU  - Waterboer T
AD  - Institute of Microbiology and Virology, University of Witten/Herdecke, Germany.
FAU - Rahaus, Markus
AU  - Rahaus M
FAU - Wolff, Manfred H
AU  - Wolff MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
RN  - EC 3.1.- (Ribonucleases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Blotting, Northern
MH  - Gene Expression Regulation, Viral/drug effects
MH  - Genes, Viral
MH  - Herpesvirus 3, Human/*genetics/metabolism
MH  - Humans
MH  - Open Reading Frames/physiology
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/biosynthesis
MH  - Ribonucleases/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Viral Proteins/biosynthesis/physiology
EDAT- 2002/04/04 10:00
MHDA- 2003/07/02 05:00
CRDT- 2002/04/04 10:00
PHST- 2002/04/04 10:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2002/04/04 10:00 [entrez]
AID - 10.1023/a:1014086004141 [doi]
PST - ppublish
SO  - Virus Genes. 2002;24(1):49-56. doi: 10.1023/a:1014086004141.