PMID- 11929923
OWN - NLM
STAT- MEDLINE
DCOM- 20020612
LR  - 20131121
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 87
IP  - 4
DP  - 2002 Apr
TI  - Characterization and comparison of the NR3A subunit of the NMDA receptor in 
      recombinant systems and primary cortical neurons.
PG  - 2052-63
AB  - Recently, we cloned and began to characterize a new N-methyl-D-aspartate receptor 
      (NMDAR) subunit, NR3A. Here we extend our earlier findings by showing that 
      recombinantly expressed NR3A in COS cells is biochemically associated with both 
      NR1 and NR2 subunits. In the oocyte or HEK 293 cell expression systems, 
      co-injection of NR3A with NR1/NR2 subunits acts in a dominant-interfering manner, 
      resulting in a decrease in NMDAR unitary conductance, decrease in Ca(2+) 
      permeability, decrease in Mg(2+) sensitivity, and slight increase in mean open 
      time compared with NR1/NR2 channels. The smaller unitary conductance channel has 
      also been observed in primary cortical neurons cultured from wild-type rodent on 
      postnatal day 8 (P8) and similarly found to be relatively insensitive to Mg(2+) 
      block. Consistent with these findings, whole cell NMDA-evoked currents are larger 
      in NR3A-deficient mice compared with wild-type mice, and this effect follows a 
      developmental pattern similar to that of NR3A protein expression on Western 
      blots, with peak expression at P8. Finally, a new longer splice variant of NR3A 
      has been cloned and found to be expressed in rodent cortical neurons by 
      single-cell RT-PCR and in situ hybridization.
FAU - Sasaki, Yasnory F
AU  - Sasaki YF
AD  - Center for Neuroscience and Aging, The Burnham Institute, La Jolla, California 
      92037, USA.
FAU - Rothe, Thomas
AU  - Rothe T
FAU - Premkumar, Louis S
AU  - Premkumar LS
FAU - Das, Saumya
AU  - Das S
FAU - Cui, Jiankun
AU  - Cui J
FAU - Talantova, Maria V
AU  - Talantova MV
FAU - Wong, Hon-Kit
AU  - Wong HK
FAU - Gong, Xiandi
AU  - Gong X
FAU - Chan, Shing Fai
AU  - Chan SF
FAU - Zhang, Dongxian
AU  - Zhang D
FAU - Nakanishi, Nobuki
AU  - Nakanishi N
FAU - Sucher, Nikolaus J
AU  - Sucher NJ
FAU - Lipton, Stuart A
AU  - Lipton SA
LA  - eng
GR  - HD-29587/HD/NICHD NIH HHS/United States
GR  - R01 EY-05477/EY/NEI NIH HHS/United States
GR  - R01 MH-53535/MH/NIMH NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Ion Channels)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Recombinant Proteins)
RN  - I38ZP9992A (Magnesium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alternative Splicing
MH  - Animals
MH  - COS Cells
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/*metabolism
MH  - Electric Conductivity
MH  - Female
MH  - Immunoblotting
MH  - Ion Channels/drug effects/physiology
MH  - Magnesium/pharmacology
MH  - Mice
MH  - Neurons/*metabolism
MH  - Oocytes
MH  - Permeability
MH  - Precipitin Tests
MH  - Protein Subunits
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Xenopus laevis
EDAT- 2002/04/04 10:00
MHDA- 2002/06/13 10:01
CRDT- 2002/04/04 10:00
PHST- 2002/04/04 10:00 [pubmed]
PHST- 2002/06/13 10:01 [medline]
PHST- 2002/04/04 10:00 [entrez]
AID - 10.1152/jn.00531.2001 [doi]
PST - ppublish
SO  - J Neurophysiol. 2002 Apr;87(4):2052-63. doi: 10.1152/jn.00531.2001.