PMID- 11930666
OWN - NLM
STAT- MEDLINE
DCOM- 20020418
LR  - 20131121
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 115
IP  - 1
DP  - 2002 Jan
TI  - Overexpression of bcl-2 protects hepatoma cell line HCC-9204 from ethanol-induced 
      apoptosis.
PG  - 8-11
AB  - OBJECTIVE: To investigate the effect of overexpression of bcl-2 on 
      ethanol-induced apoptosis of primary hepatocellular carcinoma (HCC) cells. 
      METHODS: The retrovirus expression vector pDOR-SB containing human bcl-2 cDNA was 
      introduced into a human HCC cell line HCC-9204 by liposome-mediated transfection. 
      pDOR-transfected and non-transfected HCC-9204 cells were used as controls. The 
      expression of Bcl-2 protein by transfected HCC-9204 cells was detected by the 
      immunohistochemical method. Then the cells were cloned with the limited dilution 
      method continually until a monoclonal cell strain whose positive rate of Bcl-2 
      protein was 100% detected by flow cytometry was obtained. The killing rates of 
      cells were detected by Methabenzthiazuron assay after the treatment of 6% ethanol 
      for 6 h. The extent of apoptosis was analyzed by transferase-mediated dUTP nick 
      end labeling (TUNEL) staining and flow cytometry. RESULTS: Most of the 
      pDOR-SB-transfected cells demonstrated Bcl-2 positive signals, while no signal 
      was found in the controls. The positive rate of Bcl-2 protein detected by flow 
      cytometry in the obtained monoclonal cell strain, which was named HCC-bcl2, was 
      100% after the cells had been cloned 3 times continually. The killing rate, TUNEL 
      index and the scale of sub-G1 apoptotic peak in HCC-bcl2 cells were all 
      significantly lower than those in the control cells. CONCLUSION: Overexpression 
      of Bcl-2 protein suppresses ethanol-induced apoptosis of the HCC cell line 
      HCC-9204.
FAU - Yang, Lianjun
AU  - Yang L
AD  - Department of Pathology, Fourth Military Medical University, Xi'an 710032, China. 
      yangsi@sina.com
FAU - Si, Xiaohui
AU  - Si X
FAU - Wang, Wenliang
AU  - Wang W
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Hepatocellular/metabolism/*pathology
MH  - Ethanol/*pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Liver Neoplasms/metabolism/*pathology
MH  - Proto-Oncogene Proteins c-bcl-2/*physiology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2002/04/05 10:00
MHDA- 2002/04/19 10:01
CRDT- 2002/04/05 10:00
PHST- 2002/04/05 10:00 [pubmed]
PHST- 2002/04/19 10:01 [medline]
PHST- 2002/04/05 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2002 Jan;115(1):8-11.