PMID- 11930983
OWN - NLM
STAT- MEDLINE
DCOM- 20021022
LR  - 20220309
IS  - 1124-4909 (Print)
IS  - 1124-4909 (Linking)
VI  - 7
IP  - 1
DP  - 2002 Mar
TI  - Effects of restraint stress and serotonin on macronutrient selection: a rat model 
      of stress-induced anorexia.
PG  - 23-31
AB  - The present study investigated the effects of brief (20 min), acute (2 hr) and 
      chronic restraint stress (2 hr/day for five days) at the time of dark onset on 
      macronutrient selection in female Wistar rats. The role of 5-HT1A receptors in 
      nutrient intake was also examined in a dose-response study (100-700 microg/kg 
      body weight) of 8-hydroxy-2-(di-n-propylamino) tetralin (DPAT) in an acute 
      restraint model. A total of 200 rats aged 6-12 months were tested. In all cases, 
      the restraint stress took the form of confinement within tight-fitting cylinder 
      tubes. In the brief and acute paradigms, the intake of pure carbohydrate (CHO), 
      protein (PRO), and fat was measured for 40 min after a single exposure to stress; 
      in the chronic model, nutrient intake was assessed for 40 min only after the 
      final restraint stress session on day 5. In the DPAT paradigm, intraperitoneal 
      injections of the 5-HT1A agonist were given prior to acute restraint and a 40 min 
      session of nutrient self-selection. Statistical analysis using the t-test for 
      independent samples revealed that neither PRO nor total intake was significantly 
      altered by restraint in the brief, acute or chronic restraint experiments, 
      whereas CHO consumption was suppressed by acute (p=0.02) and chronic restraint 
      (p=0.021), and fat intake was suppressed by brief (p=0.010), acute (p=0.002) and 
      chronic restraint (p=0.001). In the DPAT paradigm, acute restraint stress 
      suppressed CHO (p=0.0001), fat (p=0.000001) and total intake (p=0.003). These 
      effects were not reversed by DPAT, and the administration of 300 pg/kg actually 
      further reduced fat intake. In conclusion, fat intake is more sensitive to the 
      suppressive effects of restraint stress than CHO intake, whereas protein intake 
      is unaffected. Furthermore, 5-HT1A receptors do not seem to play a role in the 
      effects of acute restraint on CHO and fat intake.
FAU - Wang, S W J
AU  - Wang SW
AD  - Department of Behavioral Neuroscience, University of British Columbia, Vancouver, 
      Canada. stwang79@hotmail.com
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eat Weight Disord
JT  - Eating and weight disorders : EWD
JID - 9707113
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Dietary Proteins)
RN  - 0 (Receptors, Serotonin)
RN  - 0 (Receptors, Serotonin, 5-HT1)
RN  - 0 (Serotonin Receptor Agonists)
RN  - 333DO1RDJY (Serotonin)
RN  - 78950-78-4 (8-Hydroxy-2-(di-n-propylamino)tetralin)
SB  - IM
MH  - 8-Hydroxy-2-(di-n-propylamino)tetralin
MH  - Analysis of Variance
MH  - Animals
MH  - *Anorexia Nervosa/physiopathology
MH  - Brain Chemistry
MH  - Dietary Carbohydrates/administration & dosage
MH  - Dietary Fats/administration & dosage
MH  - Dietary Proteins/administration & dosage
MH  - *Energy Intake/drug effects/physiology
MH  - *Feeding Behavior/physiology/psychology
MH  - Female
MH  - Models, Animal
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Serotonin
MH  - Receptors, Serotonin, 5-HT1
MH  - *Restraint, Physical
MH  - Serotonin/*physiology
MH  - Serotonin Receptor Agonists/pharmacology
MH  - Stress, Psychological/*complications
EDAT- 2002/04/05 10:00
MHDA- 2002/10/31 04:00
CRDT- 2002/04/05 10:00
PHST- 2002/04/05 10:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2002/04/05 10:00 [entrez]
AID - 10.1007/BF03354426 [doi]
PST - ppublish
SO  - Eat Weight Disord. 2002 Mar;7(1):23-31. doi: 10.1007/BF03354426.