PMID- 11932206
OWN - NLM
STAT- MEDLINE
DCOM- 20020517
LR  - 20191025
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 28
IP  - 2
DP  - 2002 Apr
TI  - Changes in ontogenetic expression of estrogen receptor alpha and not of estrogen 
      receptor beta in the female rat reproductive tract.
PG  - 87-97
AB  - To evaluate ontogenetic expression and localization of estrogen receptor (ER) 
      alpha and beta in fetal female rat reproductive tract, competitive RT-PCR and 
      immunohistochemistry were performed. Expression levels for Mullerian ERalpha, 
      ERbeta1 and ERbeta2 mRNAs were determined by competitive RT-PCR. ERalpha 
      expression on gestational day (GD) 15 x 5 increased 4 x 4-fold by GD 21 x 5, 
      whereas both ERbeta1 and ERbeta2 gene expression were maintained at lower 
      constant levels compared with ERalpha during development. ER immunolocalization 
      was evaluated within three regions along the Mullerian duct axis; these were 
      proximal, middle and caudal, which differentiate into oviduct, uterus and upper 
      vagina respectively. Nuclear ERalpha was localized predominantly in proximal 
      Mullerian epithelium, and middle and caudal Mullerian mesenchyme on GDs 15 x 5-21 
      x 5. Staining intensity for ERalpha increased with development in all regions. 
      However, ERbeta immunoreactivity was not detected in any region during prenatal 
      life after separate staining with three different polyclonal anti-rat ERbeta 
      antibodies. These findings provide fundamental information critical for 
      clarifying the species-specific physiological roles of ER subtypes during fetal 
      development and for investigating the tissue-specific mechanisms underlying the 
      prenatal response to estrogen and estrogen receptor agonists.
FAU - Okada, A
AU  - Okada A
AD  - Safety Research Laboratories, Yamanouchi Pharmaceutical Co. Ltd, Itabashi, Tokyo 
      174-8511, Japan.
FAU - Ohta, Y
AU  - Ohta Y
FAU - Buchanan, D L
AU  - Buchanan DL
FAU - Sato, T
AU  - Sato T
FAU - Inoue, S
AU  - Inoue S
FAU - Hiroi, H
AU  - Hiroi H
FAU - Muramatsu, M
AU  - Muramatsu M
FAU - Iguchi, T
AU  - Iguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
SB  - IM
MH  - Animals
MH  - Estrogen Receptor alpha
MH  - Estrogen Receptor beta
MH  - Female
MH  - Fetus/*metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Immunohistochemistry
MH  - Male
MH  - Ovary/*metabolism
MH  - Pregnancy
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Estrogen/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Uterus/*metabolism
EDAT- 2002/04/05 10:00
MHDA- 2002/05/23 10:01
CRDT- 2002/04/05 10:00
PHST- 2002/04/05 10:00 [pubmed]
PHST- 2002/05/23 10:01 [medline]
PHST- 2002/04/05 10:00 [entrez]
AID - JME01064 [pii]
AID - 10.1677/jme.0.0280087 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 2002 Apr;28(2):87-97. doi: 10.1677/jme.0.0280087.