PMID- 11932389
OWN - NLM
STAT- MEDLINE
DCOM- 20020510
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 9
DP  - 2002 May
TI  - Identification of neutralizing and nonneutralizing epitopes in the porcine 
      reproductive and respiratory syndrome virus GP5 ectodomain.
PG  - 4241-50
AB  - After infection of swine with porcine reproductive and respiratory syndrome virus 
      (PRRSV), there is a rapid rise of PRRSV-specific nonneutralizing antibodies 
      (NNA), while neutralizing antibodies (NA) are detectable not sooner than 3 weeks 
      later. To characterize neutralizing epitopes, we selected phages from a 12-mer 
      phage display library using anti-PRRSV neutralizing monoclonal antibody (MAb) 
      ISU25-C1. In addition, phages carrying peptides recognized by swine antibodies 
      with high seroneutralizing titer were isolated after subtracting from the library 
      those clones binding to swine anti-PRRSV serum with no neutralizing activity. Two 
      epitopes located in the ectodomain of PRRSV GP5 were identified. One of these 
      epitopes, which we named epitope B, was recognized both by neutralizing MAb 
      ISU25-C1 and swine neutralizing serum (NS) but not by swine nonneutralizing serum 
      (NNS), indicating that it is a neutralizing epitope. Epitope B is sequential, 
      conserved among isolates, and not immunodominant. Antibodies directed against it 
      are detected in serum late after infection. In contrast, the other epitope, which 
      we named epitope A, is hypervariable and immunodominant. Antibodies against it 
      appear early after infection with PRRSV. This epitope is recognized by swine NNA 
      but is not recognized by either neutralizing MAb ISU25-C1 or swine NA, indicating 
      that it is not involved in PRRSV neutralization. During infection with PRRSV, 
      epitope A may act as a decoy, eliciting most of the antibodies directed to GP5 
      and delaying the induction of NA against epitope B for at least 3 weeks. These 
      results are relevant to the design of vaccines against PRRSV.
FAU - Ostrowski, M
AU  - Ostrowski M
AD  - Wolf Biotech, Lincoln, Nebraska, USA.
FAU - Galeota, J A
AU  - Galeota JA
FAU - Jar, A M
AU  - Jar AM
FAU - Platt, K B
AU  - Platt KB
FAU - Osorio, F A
AU  - Osorio FA
FAU - Lopez, O J
AU  - Lopez OJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Peptides)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
EIN - J Virol 2002 Jul;76(13):6863
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/blood/immunology
MH  - Antibodies, Viral/blood/*immunology
MH  - Epitopes/chemistry/genetics/*immunology
MH  - Immunodominant Epitopes/chemistry/genetics/immunology
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Peptides/chemical synthesis/chemistry/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Envelope Proteins/*chemistry/genetics/*immunology
PMC - PMC155073
EDAT- 2002/04/05 10:00
MHDA- 2002/05/11 10:01
PMCR- 2002/05/01
CRDT- 2002/04/05 10:00
PHST- 2002/04/05 10:00 [pubmed]
PHST- 2002/05/11 10:01 [medline]
PHST- 2002/04/05 10:00 [entrez]
PHST- 2002/05/01 00:00 [pmc-release]
AID - 1890 [pii]
AID - 10.1128/jvi.76.9.4241-4250.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 May;76(9):4241-50. doi: 10.1128/jvi.76.9.4241-4250.2002.