PMID- 11932450
OWN - NLM
STAT- MEDLINE
DCOM- 20020718
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 148
IP  - Pt 4
DP  - 2002 Apr
TI  - The Candida albicans Sup35p protein (CaSup35p): function, prion-like behaviour 
      and an associated polyglutamine length polymorphism.
PG  - 1049-1060
LID - 10.1099/00221287-148-4-1049 [doi]
AB  - The Sup35p protein of Saccharomyces cerevisiae is an essential translation factor 
      whose prion-like properties give rise to the non-Mendelian genetic element 
      [PSI(+)]. In this study the SUP35 gene from the related yeast species Candida 
      albicans has been characterized. The CaSUP35 gene encodes a protein (CaSup35p) of 
      729 aa which shows 65% amino acid identity to the S. cerevisiae Sup35p protein 
      (ScSup35p), with the C-terminal region showing greater identity (79%) than the 
      N-terminal region. The full-length CaSup35p can functionally replace ScSup35p in 
      S. cerevisiae although complementation is only complete when CaSup35p is 
      overexpressed. Complementation only requires expression of the CaSup35p C domain. 
      In S. cerevisiae the full-length CaSup35p is unable to establish a prion-like 
      aggregated state even in the presence of endogenous ScSup35p prion 'seeds', thus 
      confirming the existence of a species barrier in fungal prion propagation. 
      Subcellular localization studies in C. albicans show that although CaSup35p is 
      normally ribosome-associated, when not ribosome-associated, it does not form 
      pelletable high-molecular-mass aggregates characteristic of the ScSup35p in 
      [PSI(+)] strains. Unlike the ScSup35p, the CaSup35p N domain contains a number of 
      polyglutamine repeats although it does contain seven copies of the peptide GGYQQ 
      that is repeated in the ScSup35p N domain. Analysis of the CaSUP35 gene from 14 
      different strains of C. albicans identified four naturally occurring 
      polymorphisms associated with changes in the length of the largest of the 
      polyglutamine repeats. These findings have important implications for the 
      evolution of fungal prion genes.
FAU - Resende, Catarina
AU  - Resende C
AD  - Centro de Genetica e Biologia Molecular, Universidade de Lisboa, Portugal2.
AD  - Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, 
      UK1.
FAU - Parham, Steven N
AU  - Parham SN
AD  - Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, 
      UK1.
FAU - Tinsley, Caroline
AU  - Tinsley C
AD  - Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, 
      UK1.
FAU - Ferreira, Paulo
AU  - Ferreira P
AD  - Centro de Genetica e Biologia Molecular, Universidade de Lisboa, Portugal2.
AD  - Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, 
      UK1.
FAU - Duarte, Julio A B
AU  - Duarte JAB
AD  - Centro de Genetica e Biologia Molecular, Universidade de Lisboa, Portugal2.
FAU - Tuite, Mick F
AU  - Tuite MF
AD  - Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, 
      UK1.
LA  - eng
SI  - GENBANK/AY028660
SI  - GENBANK/AY028661
SI  - GENBANK/AY028662
SI  - GENBANK/AY028663
SI  - GENBANK/AY028664
SI  - GENBANK/AY028665
SI  - GENBANK/AY028666
SI  - GENBANK/AY028667
SI  - GENBANK/AY028668
SI  - GENBANK/AY028669
SI  - GENBANK/AY028670
SI  - GENBANK/AY028671
SI  - GENBANK/AY028672
SI  - GENBANK/AY028673
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Fungal Proteins)
RN  - 0 (Peptide Termination Factors)
RN  - 0 (Peptides)
RN  - 0 (Prions)
RN  - 0 (SUP35 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Sup35 protein, Candida albicans)
RN  - 26700-71-0 (polyglutamine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/chemistry/*genetics
MH  - Base Sequence
MH  - Candida albicans/*genetics/growth & development
MH  - DNA Primers
MH  - Fungal Proteins/chemistry/*genetics
MH  - Gene Deletion
MH  - Genetic Complementation Test
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Peptide Termination Factors
MH  - Peptides/*genetics
MH  - Plasmids
MH  - *Polymorphism, Genetic
MH  - Polymorphism, Single Nucleotide
MH  - Prions/chemistry/*genetics
MH  - Ribosomes/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 2002/04/05 10:00
MHDA- 2002/07/19 10:01
CRDT- 2002/04/05 10:00
PHST- 2002/04/05 10:00 [pubmed]
PHST- 2002/07/19 10:01 [medline]
PHST- 2002/04/05 10:00 [entrez]
AID - 10.1099/00221287-148-4-1049 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2002 Apr;148(Pt 4):1049-1060. doi: 
      10.1099/00221287-148-4-1049.