PMID- 11934201
OWN - NLM
STAT- MEDLINE
DCOM- 20020827
LR  - 20220210
IS  - 1081-597X (Print)
IS  - 1081-597X (Linking)
VI  - 18
IP  - 2
DP  - 2002 Mar-Apr
TI  - Concomitant effect of topical ubiquinone Q10 and vitamin E to prevent keratocyte 
      apoptosis after excimer laser photoablation in rabbits.
PG  - 135-9
AB  - PURPOSE: To investigate in vivo whether ubiquinone Q10 together with vitamin E 
      protects rabbit corneas from keratocyte apoptosis after excimer laser 
      irradiation. METHODS: Photorefractive keratectomy (PRK) was performed in both 
      eyes of three New Zealand white rabbits. During 3 days before surgery, each right 
      eye received four-times-daily instillation of an eye-drop solution containing 
      ubiquinone Q10 0.20% and vitamin E 0.04%; each left eye was treated with a 
      solution that did not contain ubiquinone or vitamin E. The central cornea was 
      analyzed after surgery using the in situ end labelling (ISEL) technique of nicked 
      DNA to detect DNA fragmentation. To determine the number of ISEL positive nuclei, 
      an average of 70 random microscopic fields (five for each de-epithelialized 
      tissue section) of 138,000 mu2 were examined in the right and left cornea samples 
      at 250X by two different observers. RESULTS: Light microscopic examination of the 
      sections from corneas treated before PRK showed that cells committed to apoptosis 
      by PRK were about 50% compared to those of untreated controls. CONCLUSION: 
      Treatment of rabbit eyes before PRK with ubiquinone Q10 lowered the number of 
      apoptotic events.
FAU - Brancato, Rosario
AU  - Brancato R
AD  - Department of Ophthalmology & Visual Sciences, University Hospital San Raffaele 
      of Milan, Italy. brancato.rosario@hsr.it
FAU - Fiore, Tito
AU  - Fiore T
FAU - Papucci, Laura
AU  - Papucci L
FAU - Schiavone, Nicola
AU  - Schiavone N
FAU - Formigli, Lucia
AU  - Formigli L
FAU - Orlandini, Sandra Zecchi
AU  - Orlandini SZ
FAU - Gobbi, Pier Giorgio
AU  - Gobbi PG
FAU - Carones, Francesco
AU  - Carones F
FAU - Donnini, Martino
AU  - Donnini M
FAU - Lapucci, Andrea
AU  - Lapucci A
FAU - Capaccioli, Sergio
AU  - Capaccioli S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Refract Surg
JT  - Journal of refractive surgery (Thorofare, N.J. : 1995)
JID - 9505927
RN  - 0 (Coenzymes)
RN  - 0 (Ophthalmic Solutions)
RN  - 1339-63-5 (Ubiquinone)
RN  - 1406-18-4 (Vitamin E)
RN  - 9007-49-2 (DNA)
RN  - EJ27X76M46 (coenzyme Q10)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Coenzymes
MH  - Cornea/*drug effects/surgery
MH  - Cytoprotection/*drug effects
MH  - DNA/analysis
MH  - Drug Therapy, Combination
MH  - Fibroblasts/drug effects
MH  - In Situ Nick-End Labeling
MH  - Lasers, Excimer
MH  - Ophthalmic Solutions
MH  - *Photorefractive Keratectomy
MH  - Rabbits
MH  - Ubiquinone/*analogs & derivatives/*therapeutic use
MH  - Vitamin E/*therapeutic use
EDAT- 2002/04/06 10:00
MHDA- 2002/08/28 10:01
CRDT- 2002/04/06 10:00
PHST- 2002/04/06 10:00 [pubmed]
PHST- 2002/08/28 10:01 [medline]
PHST- 2002/04/06 10:00 [entrez]
AID - 10.3928/1081-597X-20020301-06 [doi]
PST - ppublish
SO  - J Refract Surg. 2002 Mar-Apr;18(2):135-9. doi: 10.3928/1081-597X-20020301-06.