PMID- 11934440
OWN - NLM
STAT- MEDLINE
DCOM- 20020624
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 501
IP  - 1-2
DP  - 2002 Apr 25
TI  - Salmonella typhimurium mutagenicity tester strains that overexpress 
      oxygen-insensitive nitroreductases nfsA and nfsB.
PG  - 79-98
AB  - We have designed and constructed a series of plasmids that contain the major 
      and/or minor Escherichia coli nitroreductase genes, nfsA and nfsB, in different 
      combinations with R plasmid mucA/B genes and the Salmonella typhimurium OAT gene. 
      The plasmid encoded gene products are necessary for both the metabolic activation 
      of a range of structurally diverse nitrosubstituted compounds, and for mutagenic 
      translation bypass. Introduction of these plasmids into S. typhimurium TA1538 and 
      TA1535 has created several new tester strains which exhibit an extremely high 
      mutagenic sensitivity and a broad substrate specificity towards a battery of 
      nitrosubstituted test compounds that included 4-nitroquinoline-1-oxide (4-NQO), 
      nitrofurazone (NF), 1-nitropyrene (1-NP), 2-nitronaphthalene (2-NN), 
      2-nitrofluorene (2-NF), and 1,6-dinitropyrene (1,6-DNP). Our studies show that 
      the nfsA gene encodes a product that is extremely effective in the metabolic 
      activation of a range of structurally diverse nitrosubstituted compounds. Several 
      of the new tester strains are more than two orders of magnitude more sensitive to 
      nitrosubstituted compounds than the Ames tester strains TA100 or TA98. In 
      addition to enhancing mutagenic sensitivity, plasmids encoding both metabolic and 
      mutagenesis functions on a single plasmid provide considerable flexibility for 
      future mechanistic studies or tester strain development, in which it may be 
      necessary to introduce additional plasmids containing different antibiotic 
      resistance markers.
FAU - Carroll, C C
AU  - Carroll CC
AD  - Biology Department, Carleton University, 1125 Colonel By Drive, Ont., Ottawa, 
      Canada.
FAU - Warnakulasuriyarachchi, D
AU  - Warnakulasuriyarachchi D
FAU - Nokhbeh, M R
AU  - Nokhbeh MR
FAU - Lambert, I B
AU  - Lambert IB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Bacterial Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (MucA protein, Bacteria)
RN  - 0 (MucB protein, Bacteria)
RN  - 0 (Nitro Compounds)
RN  - EC 1.7.- (NfsA protein, E coli)
RN  - EC 1.7.- (NfsB protein, E coli)
RN  - EC 1.7.- (Nitroreductases)
SB  - IM
MH  - Bacterial Proteins/genetics
MH  - *Drug Resistance, Neoplasm
MH  - Escherichia coli
MH  - *Escherichia coli Proteins
MH  - *Mutagenicity Tests
MH  - Nitro Compounds/toxicity
MH  - Nitroreductases/biosynthesis/*genetics
MH  - Plasmids
MH  - Salmonella typhimurium/*genetics
MH  - Sensitivity and Specificity
EDAT- 2002/04/06 10:00
MHDA- 2002/06/25 10:01
CRDT- 2002/04/06 10:00
PHST- 2002/04/06 10:00 [pubmed]
PHST- 2002/06/25 10:01 [medline]
PHST- 2002/04/06 10:00 [entrez]
AID - S0027510702000180 [pii]
AID - 10.1016/s0027-5107(02)00018-0 [doi]
PST - ppublish
SO  - Mutat Res. 2002 Apr 25;501(1-2):79-98. doi: 10.1016/s0027-5107(02)00018-0.