PMID- 11934706
OWN - NLM
STAT- MEDLINE
DCOM- 20020517
LR  - 20110428
IS  - 1931-857X (Print)
IS  - 1522-1466 (Linking)
VI  - 282
IP  - 5
DP  - 2002 May
TI  - Identification of developmentally regulated mesodermal-specific transcript in 
      mouse embryonic metanephros.
PG  - F953-65
AB  - Mesodermal-specific cDNA or transcript (MEST) was identified by suppression 
      subtractive hybridization-PCR of cDNA isolated from embryonic day 13 vs. newborn 
      mice kidneys. At day 13 of mouse gestation, a high expression of MEST, with a 
      single approximately 2.7-kb transcript that was exclusively localized to the 
      metanephric mesenchyme was observed. The MEST mRNA expression gradually decreased 
      during the later stages and then abruptly decreased in the newborn kidneys and 
      subsequent postnatal life, after which a very mild expression persisted in the 
      glomerular mesangium. Regression in mRNA expression during embryonic renal 
      development appears to be related to methylation of the MEST gene. Treatment of 
      metanephroi, harvested at day 13 of gestation with MEST-specific antisense 
      oligodeoxynucleotide resulted in a dose-dependent decrease in the size of the 
      explants and the nephron population. This was associated with a selective 
      decrease in MEST mRNA expression and accelerated apoptosis of the mesenchyme. 
      These findings suggest that MEST, a gene with a putative mesenchymal cell-derived 
      protein, conceivably plays a role in mammalian metanephric development.
FAU - Kanwar, Yashpal S
AU  - Kanwar YS
AD  - Department of Pathology, Northwestern University Medical School, Chicago, 
      Illinois 60611, USA. y-kanwar@northwestern.edu
FAU - Kumar, Anil
AU  - Kumar A
FAU - Ota, Kosuke
AU  - Ota K
FAU - Lin, Sun
AU  - Lin S
FAU - Wada, Jun
AU  - Wada J
FAU - Chugh, Sumant
AU  - Chugh S
FAU - Wallner, Elisabeth I
AU  - Wallner EI
LA  - eng
GR  - DK28492/DK/NIDDK NIH HHS/United States
GR  - DK60635/DK/NIDDK NIH HHS/United States
GR  - DK61275/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (DNA, Complementary)
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (mesoderm specific transcript protein)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/metabolism
MH  - Apoptosis
MH  - DNA Methylation
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Gestational Age
MH  - In Situ Hybridization
MH  - In Situ Nick-End Labeling
MH  - Kidney/*embryology/growth & development/metabolism
MH  - Male
MH  - Mesoderm/*chemistry
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nucleic Acid Hybridization
MH  - Oligodeoxyribonucleotides, Antisense/pharmacology
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Proteins/*genetics
MH  - RNA, Messenger/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2002/04/06 10:00
MHDA- 2002/05/23 10:01
CRDT- 2002/04/06 10:00
PHST- 2002/04/06 10:00 [pubmed]
PHST- 2002/05/23 10:01 [medline]
PHST- 2002/04/06 10:00 [entrez]
AID - 10.1152/ajprenal.00200.2001 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2002 May;282(5):F953-65. doi: 
      10.1152/ajprenal.00200.2001.