PMID- 11936217
OWN - NLM
STAT- MEDLINE
DCOM- 20020416
LR  - 20061115
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 65
IP  - 5-6
DP  - 2002 Mar
TI  - Detection of chromosome-specific aneusomy and translocation by benzene 
      metabolites in human lymphocytes using fluorescence in situ hybridization with 
      DNA probes for chromosomes 5, 7, 8, and 21.
PG  - 365-72
AB  - Benzene is a widespread human carcinogen, inducing leukemia and hematotoxicity. 
      Exposure of human lymphocytes to benzene metabolites has been shown to cause 
      genetic damage, including aneusomy and chromosome aberrations. In order to detect 
      the specific chromosomal changes in chromosomes 5, 7, 8, and 21 induced by 
      benzene metabolites, 1,2,4-benzenetriol (BT), hydroquinone (HQ), and 
      trans,trans-muconic acid (t,t-MA), fluorescence in situ hybridization (FISH) 
      procedure in the metaphase spread of human lymphocytes was employed. Treatment 
      with BT, HQ and tt-MA resulted in the induction of monosomy 5, 7, 8, and 21 in 
      human lymphocytes in a concentration-dependent manner. All of these metabolites 
      also induced trisomy 5, 7, 8, and 21, but no correlation between frequencies of 
      trisomy and concentration was found. Translocations between chromosome 8 and 
      another unidentified chromosome [t(8:?)] and between chromosome 21 and another 
      unidentified chromosome [t(21:?)] were found. However, translocation between 
      chromosome 8 and 21 [t(8:2 1)] was not found. Results indicate that the benzene 
      metabolites BT, HQ and t,t-MA induce chromosome-specific numerical and structural 
      aberrations, and the fluorescence in situ hybridization (FISH) approach may be a 
      useful and powerful technique for detection of aneuploidy.
FAU - Chung, Hai Won
AU  - Chung HW
AD  - School of Public Health, Seoul National University, Korea. chunghw@snu.ac.kr
FAU - Kim, Su Young
AU  - Kim SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Benzene Derivatives)
RN  - 0 (Carcinogens)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Benzene Derivatives/*adverse effects
MH  - Carcinogens/*adverse effects
MH  - Cell Culture Techniques
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Chromosomes, Human, Pair 5/*genetics
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes
MH  - *Ploidies
MH  - *Translocation, Genetic
EDAT- 2002/04/09 10:00
MHDA- 2002/04/17 10:01
CRDT- 2002/04/09 10:00
PHST- 2002/04/09 10:00 [pubmed]
PHST- 2002/04/17 10:01 [medline]
PHST- 2002/04/09 10:00 [entrez]
AID - 10.1080/15287390252808037 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2002 Mar;65(5-6):365-72. doi: 
      10.1080/15287390252808037.