PMID- 11937269
OWN - NLM
STAT- MEDLINE
DCOM- 20021011
LR  - 20211203
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 30
IP  - 4
DP  - 2002 Apr
TI  - Human T-cell lymphotropic virus type I Tax activates lung resistance-related 
      protein expression in leukemic clones established from an adult T-cell leukemia 
      patient.
PG  - 340-5
AB  - OBJECTIVE: We examined the significance of human T-cell lymphotropic virus type I 
      (HTLV-I) Tax protein-induced resistance to anticancer drugs and the relationship 
      between Tax and multidrug resistance proteins. MATERIALS AND METHODS: S1T cell, a 
      leukemic non-Tax-producing T-cell clone established from an adult T-cell leukemia 
      (ATL) patient, S1TcTax05 and S1TcTax10 clones, transfected with Tax stably 
      expressing cDNA, and S1Tneo, transfected with a neomycin-resistant gene, were 
      examined for Tax-related anticancer drug resistance. Resistance of those cells to 
      the anticancer drugs doxorubicin, etoposide, cisplatin, and vindesine was tested 
      with the MTT method. Expression of multidrug resistance protein mRNAs (MDR1, 
      MRP1, cMOAT/MRP2, and LRP) was analyzed with reverse transcriptase polymerase 
      chain reaction (RT-PCR). Doxorubicin subcellular distribution in those cells was 
      examined by fluorescence microscopy. RESULTS: S1TcTax05 and S1TcTax10 showed 
      resistance to doxorubicin, etoposide, and vindesine, but not to cisplatin as 
      compared with S1T or S1Tneo. RT-PCR demonstrated that MRP1 mRNA was expressed, 
      but MDR1, cMOAT, and LRP mRNAs were not in S1T or S1Tneo. Marked expression of 
      LRP mRNA was detected, but no change of MDR1, MRP1, or cMOAT mRNA expression in 
      Tax-expressing S1TcTax05 and S1TcTax10. Fluorescence microscopy demonstrated that 
      doxorubicin was distributed mainly in the cytoplasm of S1TcTax05 and S1TcTax10, 
      and in the nucleus of S1T and S1Tneo. CONCLUSIONS: These findings suggest that 
      Tax-related drug resistance of ATL cells is due to LRP and not MDR1, as reported 
      previously. These findings in cells derived from an ATL patient suggest a novel 
      mechanism for drug resistance in Tax-expressing ATL cells.
FAU - Sakaki, Yoshimune
AU  - Sakaki Y
AD  - First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, 
      Sakuragaoka 8-35-1, Kagoshima 890-8520, Japan.
FAU - Terashi, Kenji
AU  - Terashi K
FAU - Yamaguchi, Akihiko
AU  - Yamaguchi A
FAU - Kawamata, Nakaaki
AU  - Kawamata N
FAU - Tokito, Yuichi
AU  - Tokito Y
FAU - Mori, Hiroyasu
AU  - Mori H
FAU - Umehara, Megumi
AU  - Umehara M
FAU - Yoshiyama, Takeshi
AU  - Yoshiyama T
FAU - Ohtsubo, Hideo
AU  - Ohtsubo H
FAU - Arimura, Kosei
AU  - Arimura K
FAU - Arima, Naomichi
AU  - Arima N
FAU - Tei, Chuwa
AU  - Tei C
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (ABCC2 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Gene Products, tax)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Multidrug Resistance-Associated Protein 2)
RN  - 0 (Multidrug Resistance-Associated Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vault Ribonucleoprotein Particles)
RN  - 0 (major vault protein)
RN  - Y49M64GZ4Q (multidrug resistance-associated protein 1)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics
MH  - ATP-Binding Cassette Transporters/genetics/*metabolism
MH  - Adult
MH  - Antineoplastic Agents/pharmacokinetics
MH  - Cell Compartmentation
MH  - Clone Cells/metabolism/virology
MH  - Dose-Response Relationship, Drug
MH  - Gene Products, tax/*physiology
MH  - HTLV-I Infections/*pathology/virology
MH  - Human T-lymphotropic virus 1/chemistry
MH  - Humans
MH  - *Membrane Transport Proteins
MH  - Multidrug Resistance-Associated Protein 2
MH  - Multidrug Resistance-Associated Proteins/genetics
MH  - RNA, Messenger/metabolism
MH  - Tumor Cells, Cultured
MH  - *Vault Ribonucleoprotein Particles
EDAT- 2002/04/09 10:00
MHDA- 2002/10/12 04:00
CRDT- 2002/04/09 10:00
PHST- 2002/04/09 10:00 [pubmed]
PHST- 2002/10/12 04:00 [medline]
PHST- 2002/04/09 10:00 [entrez]
AID - S0301472X02007750 [pii]
AID - 10.1016/s0301-472x(02)00775-0 [doi]
PST - ppublish
SO  - Exp Hematol. 2002 Apr;30(4):340-5. doi: 10.1016/s0301-472x(02)00775-0.