PMID- 11937571
OWN - NLM
STAT- MEDLINE
DCOM- 20020523
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 168
IP  - 8
DP  - 2002 Apr 15
TI  - Monocyte chemoattractant protein-1 and 5-lipoxygenase products recruit leukocytes 
      in response to platelet-activating factor-like lipids in oxidized low-density 
      lipoprotein.
PG  - 4112-20
AB  - Oxidized low-density lipoprotein (LDL) contains inflammatory agents, including 
      oxidatively fragmented phospholipids that activate the platelet-activating factor 
      (PAF) receptor, but in vivo events caused by these pathologically generated 
      agents are not well defined. Injection of PAF-like lipids derived from oxidized 
      LDL, or C(4)-PAF that is a major PAF-like lipid in these particles, into the 
      pleural cavity of mice resulted in rapid monocyte, neutrophil, and eosinophil 
      accumulation. Increased numbers of intracellular lipid bodies in these cells show 
      they were in an inflammatory environment. Leukocyte recruitment was abolished by 
      a PAF receptor antagonist, as expected. PAF-like lipids induced 5-lipoxygenase 
      expression in leukocytes, mRNA expression for monocyte chemoattractant protein-1 
      (MCP-1) and other chemokines, synthesis of MCP-1, and leukotriene B(4). The 
      5-lipoxygenase inhibitor zileuton impaired neutrophil influx, while MCP-1 had a 
      more global role, as determined with MCP-1(-/-) mice. The lack of MCP-1 abrogated 
      leukocyte accumulation and lipid body formation both in vivo and in vitro and 
      chemokine transcription in vivo, and reduced in vivo leukotriene B(4) production. 
      Thus, PAF-like phospholipids in oxidized LDL induce an inflammatory infiltrate 
      through the PAF receptor, chemokine transcription, lipid body formation, and 
      5-lipoxygenase expression in leukocytes. MCP-1 has a key role in this 
      inflammatory response, and 5-lipoxygenase products are essential for neutrophil 
      recruitment into the inflamed pleural cavity.
FAU - Silva, Adriana R
AU  - Silva AR
AD  - Departamento de Fisiologia e Farmacodinamica, Instituto Oswaldo Cruz, Fundacao 
      Oswaldo Cruz, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - de Assis, Edson F
AU  - de Assis EF
FAU - Caiado, Lara F C
AU  - Caiado LF
FAU - Marathe, Gopal K
AU  - Marathe GK
FAU - Bozza, Marcelo T
AU  - Bozza MT
FAU - McIntyre, Thomas M
AU  - McIntyre TM
FAU - Zimmerman, Guy A
AU  - Zimmerman GA
FAU - Prescott, Stephen M
AU  - Prescott SM
FAU - Bozza, Patricia T
AU  - Bozza PT
FAU - Castro-Faria-Neto, Hugo C
AU  - Castro-Faria-Neto HC
LA  - eng
GR  - HL44252/HL/NHLBI NIH HHS/United States
GR  - HL44513/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Diterpenes)
RN  - 0 (Ginkgolides)
RN  - 0 (Lactones)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Phospholipids)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (oxidized low density lipoprotein)
RN  - DF149B9460 (ginkgolide B)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
SB  - IM
MH  - Animals
MH  - Arachidonate 5-Lipoxygenase/biosynthesis/*physiology
MH  - Cell Movement/genetics/*immunology
MH  - Chemokine CCL2/biosynthesis/deficiency/genetics/*physiology
MH  - Chemokines/biosynthesis/genetics
MH  - Disease Models, Animal
MH  - *Diterpenes
MH  - Eosinophils/immunology/metabolism/pathology
MH  - Ginkgolides
MH  - Humans
MH  - Inflammation/enzymology/metabolism/pathology
MH  - Lactones/pharmacology
MH  - Leukocytes/*enzymology/*immunology/pathology
MH  - Lipoproteins, LDL/metabolism/*physiology
MH  - Mice
MH  - Neutrophils/immunology/metabolism/pathology
MH  - Phospholipids/isolation & purification/metabolism/*physiology
MH  - Platelet Activating Factor/antagonists & inhibitors/*physiology
MH  - Pleural Effusion/metabolism/pathology
MH  - Pleurisy/enzymology/immunology/metabolism/pathology
MH  - RNA, Messenger/biosynthesis
EDAT- 2002/04/09 10:00
MHDA- 2002/05/25 10:01
CRDT- 2002/04/09 10:00
PHST- 2002/04/09 10:00 [pubmed]
PHST- 2002/05/25 10:01 [medline]
PHST- 2002/04/09 10:00 [entrez]
AID - 10.4049/jimmunol.168.8.4112 [doi]
PST - ppublish
SO  - J Immunol. 2002 Apr 15;168(8):4112-20. doi: 10.4049/jimmunol.168.8.4112.