PMID- 11938350
OWN - NLM
STAT- MEDLINE
DCOM- 20020531
LR  - 20220227
IS  - 1529-2908 (Print)
IS  - 1529-2908 (Linking)
VI  - 3
IP  - 5
DP  - 2002 May
TI  - Lipid length controls antigen entry into endosomal and nonendosomal pathways for 
      CD1b presentation.
PG  - 435-42
AB  - CD1 proteins present various glycolipid antigens to T cells, but the cellular 
      mechanisms that control which particular glycolipids generate T cell responses 
      are not understood. We show here that T cell recognition of glucose monomycolate 
      antigens with long (C(80)) alkyl chains involves the delivery of CD1b proteins 
      and antigens to late endosomes in a process that takes several hours. In 
      contrast, analogs of the same antigen with shorter (C(32)) alkyl chains are 
      rapidly, but inefficiently, presented by cell surface CD1b proteins. Dendritic 
      cells (DCs) preferentially present long-chain glycolipids, which results, in 
      part, from their rapid internalization and selective delivery of antigens to 
      endosomal compartments. Nonprofessional antigen-presenting cells, however, 
      preferentially present short-chain glycolipids because of their lack of prominent 
      endosomal presentation pathways. Because long alkyl chain length distinguishes 
      certain microbial glycolipids from common mammalian glycolipids, these findings 
      suggest that DCs use a specialized endosomal-loading pathway to promote 
      preferential recognition of glycolipids with a more intrinsically foreign 
      structure.
FAU - Moody, D Branch
AU  - Moody DB
AD  - Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital 
      and Harvard Medical School, Boston, MA 02115, USA. bmoody@rics.bwh.harvard.edu
FAU - Briken, Volker
AU  - Briken V
FAU - Cheng, Tan-Yun
AU  - Cheng TY
FAU - Roura-Mir, Carme
AU  - Roura-Mir C
FAU - Guy, Mark R
AU  - Guy MR
FAU - Geho, David H
AU  - Geho DH
FAU - Tykocinski, Mark L
AU  - Tykocinski ML
FAU - Besra, Gurdyal S
AU  - Besra GS
FAU - Porcelli, Steven A
AU  - Porcelli SA
LA  - eng
GR  - AI 31044/AI/NIAID NIH HHS/United States
GR  - AI 38960/AI/NIAID NIH HHS/United States
GR  - AI45889/AI/NIAID NIH HHS/United States
GR  - AI48933/AI/NIAID NIH HHS/United States
GR  - AI49313/AI/NIAID NIH HHS/United States
GR  - ARO1988/AR/NIAMS NIH HHS/United States
GR  - CA74958/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20020408
PL  - United States
TA  - Nat Immunol
JT  - Nature immunology
JID - 100941354
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1b antigen)
RN  - 0 (Glycolipids)
RN  - 0 (Interleukin-2)
RN  - 0 (glucose mycolate)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
SB  - IM
CIN - Nat Immunol. 2002 May;3(5):421-2. PMID: 11976717
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD1/*immunology
MH  - Dendritic Cells/immunology/metabolism
MH  - Endosomes/*immunology/metabolism
MH  - Glycolipids/*immunology
MH  - Humans
MH  - Interleukin-2/analysis/biosynthesis
MH  - Lysosomes/immunology
MH  - Structure-Activity Relationship
MH  - T-Lymphocytes/immunology
MH  - Tumor Cells, Cultured
MH  - beta-N-Acetylhexosaminidases/analysis/biosynthesis
EDAT- 2002/04/09 10:00
MHDA- 2002/06/01 10:01
CRDT- 2002/04/09 10:00
PHST- 2002/04/09 10:00 [pubmed]
PHST- 2002/06/01 10:01 [medline]
PHST- 2002/04/09 10:00 [entrez]
AID - ni780 [pii]
AID - 10.1038/ni780 [doi]
PST - ppublish
SO  - Nat Immunol. 2002 May;3(5):435-42. doi: 10.1038/ni780. Epub 2002 Apr 8.