PMID- 11938951
OWN - NLM
STAT- MEDLINE
DCOM- 20021226
LR  - 20181130
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 33
IP  - 2
DP  - 1998
TI  - [Determination of 6 isoflavonoids in the hairy root cultures of Astragalus 
      membranaceus by HPLC].
PG  - 148-51
AB  - An accurate RP-HPLC method for the determination of 10-hydroxy-3, 
      9-dimethoxypeterocarpan, (3R) 8,2'-dihydroxy-7,4'-dimethoxyisoflavan, 
      formonenetin (7-hydroxy-4'-methoxyisoflavone), 8, 3'-dihydroxy-7, 
      4'-dimethoxy-isoflavone, 2'-hydroxy-3', 4'-dimethoxyisoflavan-7-O-glucopyranoside 
      and alycosin (7,3'-dihydroxy-4'-methoxyisoflavone) in the hairy root cultures of 
      Astragalus membranaceus was developed. Among them, 10-hydroxy-3, 
      9-dimethoxypeterocarpan, (3R) 8,2'-dihydroxy-7,4'-dimethoxyisoflavan and 
      8,3'-dihydroxy-7,4'-dimethoxyisoflavone were new compounds in Astragalus 
      membranaceus. The analytical column was Nucleosil C18. The mobile phases were 
      methanol-water = 3:2 and 1:1 (v/v). The detection wavelengths were 254 nm and 280 
      nm. Calibration graphs were rectilinear between 2.5 micrograms and 12.5 
      micrograms. The recoveries were between 96.47% and 103.33%. The relative standard 
      deviations (RSDs) of measurement precision test were 2.57%-6.52%. The flavonoid 
      contents in the hairy root cultures were between 0.0005% and 0.0065% (dry 
      weight).
FAU - Zheng, Z
AU  - Zheng Z
AD  - Laboratory of Biotechnology of Chinese Materia Medica, Shanghai University of 
      Traditional Chinese Medicine and Pharmacy, Shanghai 200032.
FAU - Song, C
AU  - Song C
FAU - Liu, D
AU  - Liu D
FAU - Hu, Z
AU  - Hu Z
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (Isoflavones)
SB  - IM
MH  - Astragalus propinquus/*chemistry/growth & development
MH  - Chromatography, High Pressure Liquid/methods
MH  - Culture Techniques
MH  - Isoflavones/*analysis
MH  - Plant Roots/chemistry/growth & development
EDAT- 2002/04/10 10:00
MHDA- 2002/12/27 04:00
CRDT- 2002/04/10 10:00
PHST- 2002/04/10 10:00 [pubmed]
PHST- 2002/12/27 04:00 [medline]
PHST- 2002/04/10 10:00 [entrez]
PST - ppublish
SO  - Yao Xue Xue Bao. 1998;33(2):148-51.