PMID- 11940069
OWN - NLM
STAT- MEDLINE
DCOM- 20020919
LR  - 20190901
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 32
IP  - 3
DP  - 2002 Mar
TI  - Effect of ozone exposure on allergic sensitization and airway inflammation 
      induced by dendritic cells.
PG  - 391-6
AB  - BACKGROUND: Epidemiological studies suggest that ozone exposure is related to 
      increased asthma symptoms. Dendritic cells (DCs) are the principal 
      antigen-presenting cells in the airways. OBJECTIVE: We have examined whether 
      ambient doses of ozone (100 ppb for 2 h) enhance allergic sensitization and/or 
      airway inflammation in a mouse model. METHODS: C57BL/6 mice were sensitized to 
      inhaled ovalbumin (OVA) by intratracheal instillation of OVA-pulsed DCs on day 0. 
      Daily exposure to OVA aerosol on days 14-20 resulted in an eosinophilic airway 
      inflammation, as reflected in bronchoalveolar lavage fluid and lung histology. In 
      a first experiment, mice were exposed to ozone or room air immediately prior to 
      and following sensitization. Subsequently, we tested the effect of ozone exposure 
      during antigen challenge in DC-sensitized mice. RESULTS: Exposure to ozone during 
      sensitization did not influence airway inflammation after subsequent allergen 
      challenge. In contrast, in sensitized mice, challenge with OVA together with 
      ozone (days 14-20) resulted in enhanced airway eosinophilia and lymphocytosis, as 
      compared with mice exposed to OVA and room air (1.91 x 106 +/- 0.46 x 106 vs. 
      0.16 x 106 +/- 0.06 x 106 eosinophils/mL lavage fluid; P = 0.015; 0.49 x 106 +/- 
      0.11 x 106 vs. 0.08 x 106 +/- 0.03 x 106 lymphocytes/mL lavage fluid; P = 0.004). 
      Ozone exposure without subsequent OVA exposure did not cause airway inflammation. 
      CONCLUSION: Ozone exposure does not increase allergic sensitization but enhances 
      antigen-induced airway inflammation in mice that are sensitized via the airways.
FAU - Depuydt, P O
AU  - Depuydt PO
AD  - Department of Respiratory Diseases, Ghent University Hospital, Ghent, Belgium. 
      guy.joos@rug.ac.be
FAU - Lambrecht, B N
AU  - Lambrecht BN
FAU - Joos, G F
AU  - Joos GF
FAU - Pauwels, R A
AU  - Pauwels RA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Air Pollutants)
RN  - 0 (Oxidants, Photochemical)
RN  - 66H7ZZK23N (Ozone)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Air Pollutants/*adverse effects/*immunology
MH  - Animals
MH  - Antigen-Presenting Cells/immunology
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Cell Movement
MH  - Dendritic Cells/*immunology/metabolism
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Immunologic
MH  - *Immunization
MH  - Inflammation/*etiology/immunology/pathology
MH  - Lung/*blood supply/cytology/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/adverse effects/immunology
MH  - Oxidants, Photochemical/*adverse effects
MH  - Ozone/*adverse effects/*immunology
MH  - Respiratory Hypersensitivity/etiology/immunology/pathology
EDAT- 2002/04/10 10:00
MHDA- 2002/09/20 10:01
CRDT- 2002/04/10 10:00
PHST- 2002/04/10 10:00 [pubmed]
PHST- 2002/09/20 10:01 [medline]
PHST- 2002/04/10 10:00 [entrez]
AID - 1364 [pii]
AID - 10.1046/j.1365-2222.2002.01364.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2002 Mar;32(3):391-6. doi: 10.1046/j.1365-2222.2002.01364.x.