PMID- 11940233 OWN - NLM STAT- MEDLINE DCOM- 20020430 LR - 20190818 IS - 0300-9475 (Print) IS - 0300-9475 (Linking) VI - 55 IP - 3 DP - 2002 Mar TI - Differential expression of neurotrophic factors and inflammatory cytokines by myelin basic protein-specific and other recruited T cells infiltrating the central nervous system during experimental autoimmune encephalomyelitis. PG - 264-73 AB - Recent evidence suggests that autoimmune reactions in the central nervous system (CNS) not only have detrimental consequences but can also be neuroprotective, and that this effect is mediated by the expression of neuronal growth factors by infiltrating leucocytes. Here we dissect these two phenomena in guinea pig myelin basic protein peptide (gpMBP 63-88)-induced experimental autoimmune encephalomyelitis (EAE) in the Lewis rat. Real-time TaqMan polymerase chain reaction (PCR) was used to measure mRNA for the nerve growth factors, brain-derived neurotrophic factor (BDNF) and neurotrophin (NT)-3. As reference, the well-known proinflammatory mediator molecules interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha were quantified. In whole lumbar cord tissue, both the nerve growth factors and the proinflammatory cytokines, IFN-gamma and TNF-alpha, displayed similar expression patterns, peaking at the height of the disease. Among the infiltrating inflammatory cells isolated and sorted from the CNS, alphabeta+/T-cell receptor (TCR)BV8S2+, but not alphabeta+/TCRBV8S2-, recognized the encephalitogenic MBP peptide. Interestingly, these two populations displayed contrasting expression patterns of nerve growth factors and proinflammatory cytokines with higher inflammatory cytokine mRNA levels in alphabeta+/TCRBV8S2+ cells at all time intervals, whereas the levels of BDNF and NT3 were higher in alphabeta+/TCRBV8S2- cells. We conclude that a potentially important neuroprotective facet of CNS inflammation dominantly prevails within other non-MBP peptide-specific lymphoid cells and that there are independent regulatory mechanisms for neurotrophin and inflammatory cytokine expression during EAE. FAU - Muhallab, S AU - Muhallab S AD - Neuroimmunology Unit, Department of Medicine, Center for Molecular Medicine L8:04, Karolinska Hospital, 17176 Stockholm, Sweden. muhallab.saad@cmm.ki.se FAU - Lundberg, C AU - Lundberg C FAU - Gielen, A W AU - Gielen AW FAU - Lidman, O AU - Lidman O FAU - Svenningsson, A AU - Svenningsson A FAU - Piehl, F AU - Piehl F FAU - Olsson, T AU - Olsson T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Scand J Immunol JT - Scandinavian journal of immunology JID - 0323767 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cytokines) RN - 0 (Inflammation Mediators) RN - 0 (Myelin Basic Protein) RN - 0 (Neurotrophin 3) RN - 0 (Peptide Fragments) RN - 0 (RNA, Messenger) RN - 0 (Tumor Necrosis Factor-alpha) RN - 82115-62-6 (Interferon-gamma) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Brain-Derived Neurotrophic Factor/*genetics MH - Central Nervous System/immunology MH - Cytokines/*genetics MH - Encephalomyelitis, Autoimmune, Experimental/genetics/*immunology MH - Female MH - Gene Expression MH - Guinea Pigs MH - Inflammation Mediators/immunology MH - Interferon-gamma/genetics MH - Molecular Sequence Data MH - Myelin Basic Protein/genetics/immunology MH - Neurotrophin 3/*genetics MH - Peptide Fragments/genetics/immunology MH - RNA, Messenger/genetics/metabolism MH - Rats MH - Rats, Inbred Lew MH - T-Lymphocytes/*immunology MH - Tumor Necrosis Factor-alpha/genetics EDAT- 2002/04/10 10:00 MHDA- 2002/05/01 10:01 CRDT- 2002/04/10 10:00 PHST- 2002/04/10 10:00 [pubmed] PHST- 2002/05/01 10:01 [medline] PHST- 2002/04/10 10:00 [entrez] AID - 1038 [pii] AID - 10.1046/j.0300-9475.2002.01038.x [doi] PST - ppublish SO - Scand J Immunol. 2002 Mar;55(3):264-73. doi: 10.1046/j.0300-9475.2002.01038.x.