PMID- 11941582
OWN - NLM
STAT- MEDLINE
DCOM- 20020529
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 19
IP  - 2
DP  - 2002 Apr
TI  - [Construction and expression of APP(SWE) transgenic mice model].
PG  - 104-7
AB  - OBJECTIVE: To prepare transgenic mice model of human Swedish mutation of amyloid 
      precursor protein(APP(SWE)) gene. METHODS: By pronuclear microinjection, human 
      APP (SWE) genes were microinjected into pronuclei of zygotes of C57 and Kunming 
      white mice. Integral zygotes were implanted into the oviducts of pseudopregnant 
      female mice. The integration and expression of exogeneous genes in offsprings 
      were detected by polymerase chain reaction (PCR), fluorescence in situ 
      hybridization (FISH) and reverse transcription-ploymerase chain reaction (RT-PCR) 
      analysis. RESULTS: The success rate of microinjection and the birth rate were 
      76.62% and 10.38%, respectively. There were 6 founders and the integration rate 
      was 35.29%. The exogeneous gene was transferred to the third generation. 
      Expression of human APP(SWE) gene was detected in the brain, heart and muscle but 
      not detected in the liver and kidney of transgenic mice. CONCLUSION: The 
      transgenic mice model of human APP(SWE) gene has been prepared successfully.
FAU - Liu, Wei
AU  - Liu W
AD  - Human Reproductive Engineering Laboratory, Xiangya Medical College, Central-South 
      University, Changsha, Hunan, 410078 P. R. China. zhuyanszzx@sina.com
FAU - Lu, Guangxiu
AU  - Lu G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Amyloid beta-Protein Precursor)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*genetics
MH  - Animals
MH  - Brain/metabolism
MH  - Cloning, Molecular
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Muscles/metabolism
MH  - Myocardium/metabolism
MH  - Plasmids/genetics
EDAT- 2002/04/10 10:00
MHDA- 2002/05/30 10:01
CRDT- 2002/04/10 10:00
PHST- 2002/04/10 10:00 [pubmed]
PHST- 2002/05/30 10:01 [medline]
PHST- 2002/04/10 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Apr;19(2):104-7.