PMID- 11943363
OWN - NLM
STAT- MEDLINE
DCOM- 20020719
LR  - 20191105
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 50
IP  - 1
DP  - 2002 Jun
TI  - A filtration, incubation and staining reactor including a new protocol for FISH.
PG  - 97-100
AB  - A newly developed device for performing fluorescence in situ hybridization (FISH) 
      is described. An adapted procedure was compared with two typical FISH protocols. 
      Tests were performed with Pseudomonas cells and the gene probe EUB338. With the 
      novel procedure, we obtained a better recovery of cells and less variability in 
      results.
FAU - Poschen, Lothar
AU  - Poschen L
AD  - Forschungszentrum Julich, Institut fur Chemie und Dynamik der Geosphare (ICG), 
      Institut IV: Agrosphare, Germany. l.poschen@fz-juelich.de
FAU - Klauth, Peter
AU  - Klauth P
FAU - Groeneweg, Joost
AU  - Groeneweg J
FAU - Wilhelm, Ralf
AU  - Wilhelm R
LA  - eng
PT  - Comparative Study
PT  - Research Support, Non-U.S. Gov't
PT  - Technical Report
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Molecular Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Artifacts
MH  - Filtration/*methods
MH  - In Situ Hybridization, Fluorescence/*instrumentation/*methods
MH  - Molecular Probes
MH  - Pseudomonas/genetics
MH  - RNA, Bacterial/*analysis
MH  - RNA, Ribosomal, 16S/*analysis
MH  - Reproducibility of Results
MH  - Staining and Labeling/methods
EDAT- 2002/04/12 10:00
MHDA- 2002/07/20 10:01
CRDT- 2002/04/12 10:00
PHST- 2002/04/12 10:00 [pubmed]
PHST- 2002/07/20 10:01 [medline]
PHST- 2002/04/12 10:00 [entrez]
AID - S0167701202000143 [pii]
AID - 10.1016/s0167-7012(02)00014-3 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2002 Jun;50(1):97-100. doi: 10.1016/s0167-7012(02)00014-3.