PMID- 11943610
OWN - NLM
STAT- MEDLINE
DCOM- 20020612
LR  - 20191210
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 516
IP  - 1-2
DP  - 2002 Apr 26
TI  - A combination of the micronucleus assay and a FISH technique for evaluation of 
      the genotoxicity of 1,2,4-benzenetriol.
PG  - 49-56
AB  - The cytokinesis-block micronucleus (CBMN) assay has emerged as one of the 
      preferred methods for assessing chromosome damage. Micronuclei (MN) are small, 
      extranuclear bodies that are formed in mitosis from acentric chromosomal 
      fragments or chromosomes that are not included in each daughter nucleus. Thus, MN 
      contain either chromosomal fragments or whole chromosomes. The CBMN assay, 
      together with a fluorescence in situ hybridization (FISH) technique using 
      specific centromeric probes for chromosomes 7 and 8, were employed in 
      mitogen-stimulated human lymphocytes pretreated with the benzene metabolite, 
      1,2,4-benzenetriol (BT). Treatment of human lymphocytes resulted in the induction 
      of MN in a dose-dependent manner. The frequency of MN in control lymphocytes was 
      4.5 per 1000 binucleated (BN) cells and this increased to 9.5, 14, 28 and 40 per 
      1000 BN cells at 10, 25, 50 and 100 microM BT, respectively. The frequency of 
      aneuploidy 7 and 8 in BN cells also increased at each concentration. Aneuploidy 8 
      was more frequent than aneuploidy 7, suggesting that chromosome 8 is more 
      sensitive to aneuploidy induction by BT. The frequency of MN containing 
      centromere positive signals for chromosomes 7 and 8 increased with the 
      concentration of BT. The frequency of MN with centromere positive signals was 
      higher for chromosome 8 than for chromosome 7, also suggesting a greater 
      sensitivity of chromosome 8 to this agent. These results suggest that combined 
      application of the CBMN assay with a FISH technique, using chromosome-specific 
      centromeric probes, would allow the detection of aneuploidy in human lymphocytes 
      and identify the mechanistic origin of MN induced by a clastogen or aneugen.
FAU - Chung, Hai Won
AU  - Chung HW
AD  - School of Public Health, Seoul National University, 28 Yunkeun-dong, Chongno-ku, 
      110-460, South Korea. chunghw@snu.ac.kr
FAU - Kang, Su Jin
AU  - Kang SJ
FAU - Kim, Su Young
AU  - Kim SY
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA Probes)
RN  - 0 (Hydroquinones)
RN  - 173O8B04RD (hydroxyhydroquinone)
SB  - IM
MH  - Aneuploidy
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 7/drug effects
MH  - Chromosomes, Human, Pair 8/drug effects
MH  - DNA Probes
MH  - Humans
MH  - Hydroquinones/*toxicity
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*drug effects
MH  - Micronuclei, Chromosome-Defective/*drug effects
MH  - *Micronucleus Tests
MH  - Mutagenicity Tests
EDAT- 2002/04/12 10:00
MHDA- 2002/06/13 10:01
CRDT- 2002/04/12 10:00
PHST- 2002/04/12 10:00 [pubmed]
PHST- 2002/06/13 10:01 [medline]
PHST- 2002/04/12 10:00 [entrez]
AID - S1383571802000189 [pii]
AID - 10.1016/s1383-5718(02)00018-9 [doi]
PST - ppublish
SO  - Mutat Res. 2002 Apr 26;516(1-2):49-56. doi: 10.1016/s1383-5718(02)00018-9.