PMID- 11945089
OWN - NLM
STAT- MEDLINE
DCOM- 20020807
LR  - 20061115
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 23
IP  - 2-3
DP  - 2002 Feb-Mar
TI  - Expression of apoptosis in placentae from mice lacking the prostaglandin F 
      receptor.
PG  - 215-23
AB  - This study aimed to investigate the changes in apoptosis in the placenta and 
      decidua of pregnant mice lacking the prostaglandin F receptor. Mouse placentae 
      were removed from fetuses on days 10-23 of pregnancy. Apoptotic cells were 
      examined by a DNA fragmentation assay and the terminal deoxynucleotidyl 
      transferase-mediated dUDP nick end-labelling (TUNEL) technique. The placenta and 
      decidual weight increased before day 18 and 14 of pregnancy, and then decreased 
      with gestational day. After day 19, the fetuses gradually died in the uterus. All 
      fetuses died in the uterus on day 23 of pregnancy. The number of apoptosis was 
      not significantly different between wild type and FP-deficient mice before day 18 
      of pregnancy by DNA fragmentation and TUNEL staining. The DNA fragmentation was 
      always more pronounced in decidual tissue on each day of pregnancy. DNA laddering 
      on placentae was more extensive on day 22 than day 18. In placenta, most 
      TUNEL-positive cells were detected in trophoblast and stromal cells. A higher 
      intensity of apoptotic cells was in the decidual basalis. The main area was the 
      centre of the decidual basalis, and was in decrease toward to margin of placenta. 
      The index of TUNEL positive cells increased as gestation progressed toward 
      termination. Especially, it was prominent in the placentae on day 22 compared 
      with that day 18 of pregnancy. The increased TUNEL-positive staining in 
      syncytiotrophoblast surface was found in placenta at post-term, compared with 
      those at term. Apoptosis may provide insights into both normal placental 
      development and placental dysfunction during an abnormal pregnancy from post-term 
      pregnancy.
CI  - Copyright 2002 Elsevier Science Ltd.
FAU - Mu, J
AU  - Mu J
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Osaka University, 
      2-2 Yamadaoka Suita, Osaka, 565-0871 Japan. mujunwu@hotmail.com
FAU - Kanzaki, T
AU  - Kanzaki T
FAU - Tomimatsu, T
AU  - Tomimatsu T
FAU - Fukuda, H
AU  - Fukuda H
FAU - Wasada, K
AU  - Wasada K
FAU - Fujii, E
AU  - Fujii E
FAU - Endoh, M
AU  - Endoh M
FAU - Kozuki, M
AU  - Kozuki M
FAU - Murata, Y
AU  - Murata Y
FAU - Sugimoto, Y
AU  - Sugimoto Y
FAU - Ichikawa, A
AU  - Ichikawa A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (prostaglandin F2alpha receptor)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Cell Count
MH  - DNA/analysis
MH  - DNA Fragmentation
MH  - Decidua/*metabolism/pathology
MH  - Female
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Organ Size
MH  - Placenta/*metabolism/pathology
MH  - Pregnancy
MH  - Receptors, Prostaglandin/*deficiency/genetics
EDAT- 2002/04/12 10:00
MHDA- 2002/08/08 10:01
CRDT- 2002/04/12 10:00
PHST- 2002/04/12 10:00 [pubmed]
PHST- 2002/08/08 10:01 [medline]
PHST- 2002/04/12 10:00 [entrez]
AID - S0143400401907593 [pii]
AID - 10.1053/plac.2001.0759 [doi]
PST - ppublish
SO  - Placenta. 2002 Feb-Mar;23(2-3):215-23. doi: 10.1053/plac.2001.0759.