PMID- 11948465
OWN - NLM
STAT- MEDLINE
DCOM- 20020501
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 98
IP  - 6
DP  - 2002 Apr 20
TI  - Expression of interleukin-18 in human ovarian carcinoma and normal ovarian 
      epithelium: evidence for defective processing in tumor cells.
PG  - 873-8
AB  - Interleukin-18 (IL-18) is a proinflammatory monokine structurally related to 
      IL-1beta that stimulates interferon-gamma (IFN-gamma) production. IL-18 is 
      synthesized as an inactive precursor, pro-IL-18, which is cleaved by 
      IL-1beta-converting enzyme (ICE)/caspase-1 in a mature protein. In view of the 
      proposed use of IL-18 in cancer immuno/gene therapy, we have studied the 
      expression of IL-18 in tumor cells. IL-18 mRNA was detected by reverse 
      transcriptase polymerase chain reaction in all human ovarian carcinoma cell lines 
      tested (9/9) and in one-half of tumor cell populations obtained from ovarian 
      carcinoma patients (4/8). ICE mRNA was expressed in a smaller fraction of samples 
      (3/9 cell lines and 3/8 samples from patients). IL-18 protein was also found in 
      7/13 ovarian carcinoma solid tumors by immunohistochemic analysis. In tumor cell 
      lines we were able to detect abundant intracellular pro-IL-18 (24 kDa) by Western 
      blotting, whereas the mature form of IL-18 was undetectable, irrespective of the 
      presence of ICE mRNA and protein. Only pro-IL-18 was also found in the ovarian 
      carcinoma cell supernatants, which did not display any IL-18 biologic activity in 
      functional assays. Normal cultured ovarian epithelial cells revealed the presence 
      of both IL-18 and ICE mRNA in all samples (5/5) and IL-18 protein was expressed 
      by the thin epithelial cell layer surrounding normal ovary. More importantly, 
      normal ovarian epithelial cells released low but detectable amounts of mature 
      IL-18 in the culture supernatant, which displayed IL-18-like biologic activity in 
      functional assays. These data suggest that mature biologically active IL-18 
      production is a feature of the normal ovarian surface epithelium lost during 
      neoplastic transformation.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Wang, Zhao Yuan
AU  - Wang ZY
AD  - Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
FAU - Gaggero, Alessia
AU  - Gaggero A
FAU - Rubartelli, Anna
AU  - Rubartelli A
FAU - Rosso, Ombretta
AU  - Rosso O
FAU - Miotti, Silvia
AU  - Miotti S
FAU - Mezzanzanica, Delia
AU  - Mezzanzanica D
FAU - Canevari, Silvana
AU  - Canevari S
FAU - Ferrini, Silvano
AU  - Ferrini S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Blotting, Western
MH  - Carcinoma, Endometrioid/genetics/*metabolism/pathology
MH  - Caspase 1/genetics/metabolism
MH  - Cells, Cultured
MH  - Cystadenocarcinoma, Serous/genetics/*metabolism/pathology
MH  - DNA Primers/chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukin-18/genetics/*metabolism
MH  - Ovarian Neoplasms/genetics/*metabolism/pathology
MH  - Ovary/*metabolism
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2002/04/12 10:00
MHDA- 2002/05/02 10:01
CRDT- 2002/04/12 10:00
PHST- 2002/04/12 10:00 [pubmed]
PHST- 2002/05/02 10:01 [medline]
PHST- 2002/04/12 10:00 [entrez]
AID - 10.1002/ijc.10268 [pii]
AID - 10.1002/ijc.10268 [doi]
PST - ppublish
SO  - Int J Cancer. 2002 Apr 20;98(6):873-8. doi: 10.1002/ijc.10268.