PMID- 11948662
OWN - NLM
STAT- MEDLINE
DCOM- 20020503
LR  - 20191210
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 68
IP  - 2
DP  - 2002 Apr 15
TI  - Phosphatidylinositol kinase enzymes regulate the retrograde axonal transport of 
      NT-3 and NT-4 in sympathetic and sensory neurons.
PG  - 169-75
AB  - Phosphatidylinositol 3-kinase (PI3-kinase) and phosphatidylinositol 4-kinase 
      (PI4-kinase) enzymes are an important family of signaling molecules that have 
      been implicated in the regulation of intracellular vesicle trafficking. It has 
      previously been shown that PI3-kinase and PI4-kinase enzymes regulate neuronal 
      survival and the retrograde axonal transport of nerve growth factor in 
      sympathetic and sensory neurons. We have extended these studies to examine the 
      role these enzymes play in the regulation of the retrograde axonal transport of 
      neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) in sympathetic and sensory 
      neurons in vivo. Wortmannin (0.1 nmol/eye), a PI3-kinase and PI4-kinase 
      antagonist, reduced the amount of (125)I-NT-3 retrograde transport in sympathetic 
      neurons by approximately 50% and (125)I-NT-4 in sympathetic neurons by 
      approximately 40% and sensory neurons by approximately 20%. The PI3-kinase 
      antagonist LY294002 (100 nmol/eye) reduced the retrograde axonal transport of 
      (125)I-NT-4 in sympathetic and sensory neurons, and (125)I-NT-3 in sympathetic 
      neurons. Phenylarsine oxide (PAO), a PI4-kinase antagonist, significantly 
      inhibited (125)I-NT-4 retrograde axonal transport in sympathetic and sensory 
      neurons. These results show that wortmannin-sensitive PI3-kinases and PI4-kinases 
      may be involved in NT-3 and NT-4 retrograde axonal transport. The retrograde 
      axonal transport of neurotrophic factors in sympathetic and sensory neurons in 
      vivo appears to depend upon the activation of different receptors and second 
      messenger cascades at the nerve terminal.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Bartlett, Selena E
AU  - Bartlett SE
AD  - Division of Neuroscience, The John Curtin School of Medical Research, Australian 
      National University, Canberra, ACT 2601, Australia.
FAU - Reynolds, Anna J
AU  - Reynolds AJ
FAU - Weible, Michael 2nd
AU  - Weible M 2nd
FAU - Hendry, Ian A
AU  - Hendry IA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (AG-879)
RN  - 0 (Androstadienes)
RN  - 0 (Arsenicals)
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Morpholines)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Tyrphostins)
RN  - 0HUR2WY345 (oxophenylarsine)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - P658DCA9XD (neurotrophin 4)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Arsenicals/pharmacology
MH  - Axonal Transport/drug effects/*physiology
MH  - Biological Transport, Active/drug effects/*physiology
MH  - Chromones/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Iris/innervation
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Morpholines/pharmacology
MH  - Nerve Growth Factors/metabolism
MH  - Neurons/*metabolism
MH  - Neurons, Afferent/*metabolism
MH  - Neurotrophin 3/metabolism
MH  - Phosphatidylinositol 3-Kinases/*physiology
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Receptor, trkA/antagonists & inhibitors
MH  - Sympathetic Nervous System/cytology/*metabolism
MH  - Tyrphostins/pharmacology
MH  - Wortmannin
EDAT- 2002/04/12 10:00
MHDA- 2002/05/04 10:01
CRDT- 2002/04/12 10:00
PHST- 2002/04/12 10:00 [pubmed]
PHST- 2002/05/04 10:01 [medline]
PHST- 2002/04/12 10:00 [entrez]
AID - 10.1002/jnr.10201 [pii]
AID - 10.1002/jnr.10201 [doi]
PST - ppublish
SO  - J Neurosci Res. 2002 Apr 15;68(2):169-75. doi: 10.1002/jnr.10201.