PMID- 11949821
OWN - NLM
STAT- MEDLINE
DCOM- 20021008
LR  - 20190910
IS  - 0167-594X (Print)
IS  - 0167-594X (Linking)
VI  - 56
IP  - 1
DP  - 2002 Jan
TI  - MCP-1 expression in CNS-1 astrocytoma cells: implications for macrophage 
      infiltration into tumors in vivo.
PG  - 1-12
AB  - Gliomas are among the most resistant tumors to conventional anti-tumor therapy, 
      and are typified by their highly infiltrative nature and ill-defined borders. 
      Macrophages constitute a major proportion of the tumor cell mass in both primary 
      human gliomas and as shown here, a CNS-1 glioma model. The objective of this 
      study was to identify tumor-cell-derived chemotactic factor(s) which participate 
      in macrophage recruitment into tumors in vivo. This study demonstrates the 
      constitutive expression of monocyte chemoattractant protein-1 (MCP-1), a potent 
      monocyte chemoattractant, by the rat astrocytoma cell line CNS-1. 
      Characterization of cytokine expression by CNS-1 cells in vitro revealed the 
      constitutive expression of TGF-beta but not other proinflammatory cytokines. 
      However, numerous cytokines were detected in CNS-I tumors in vivo including 
      Ltbeta, IL-1alpha, IL-1beta, TNF-alpha, TNF-beta, IL-10, and IFN-gamma. 
      Attenuation of MCP- I release from CNS-1 cells using an anti-sense approach 
      revealed no significant alterations in macrophage infiltration into tumors in 
      vivo, suggesting redundancy in the signal(s) involved in macrophage recruitment. 
      Depletion of peripheral macrophages using liposome-encapsulated clodronate 
      revealed no significant differences in tumor growth or in the degree of 
      macrophage infiltration into CNS-1 tumors in vivo. These results indicate that 
      CNS-1 cells produce chemotactic factors which likely participate in macrophage 
      recruitment into tumors in vivo. Whether or not macrophage recruitment confers a 
      growth advantage for the tumor remains to be determined.
FAU - Kielian, Tammy
AU  - Kielian T
AD  - Department of Pathology, Dartmouth Medical School, Dartmouth Hitchcock Medical 
      Center, Lebanon, NH, USA. kieliantammyl@uams.edu
FAU - van Rooijen, Nico
AU  - van Rooijen N
FAU - Hickey, William F
AU  - Hickey WF
LA  - eng
GR  - 5T32 AR07576/AR/NIAMS NIH HHS/United States
GR  - NS-27321/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (DNA, Antisense)
SB  - IM
MH  - Animals
MH  - Astrocytoma/*immunology/pathology
MH  - Brain Neoplasms/*immunology/pathology
MH  - Chemokine CCL2/*genetics
MH  - Cytokines/genetics
MH  - DNA, Antisense/pharmacology
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/immunology
MH  - Macrophages/cytology/*immunology
MH  - Monocytes/cytology/immunology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Tumor Cells, Cultured/immunology/transplantation
EDAT- 2002/04/13 10:00
MHDA- 2002/10/09 04:00
CRDT- 2002/04/13 10:00
PHST- 2002/04/13 10:00 [pubmed]
PHST- 2002/10/09 04:00 [medline]
PHST- 2002/04/13 10:00 [entrez]
AID - 10.1023/a:1014495613455 [doi]
PST - ppublish
SO  - J Neurooncol. 2002 Jan;56(1):1-12. doi: 10.1023/a:1014495613455.