PMID- 11951050
OWN - NLM
STAT- MEDLINE
DCOM- 20020701
LR  - 20190910
IS  - 0300-4864 (Print)
IS  - 0300-4864 (Linking)
VI  - 30
IP  - 5
DP  - 2001 May
TI  - Proliferation and death of cultured fetal neocortical neurons: effects of ethanol 
      on the dynamics of cell growth.
PG  - 391-401
AB  - Neuronal number in the mature CNS is determined by the balance of cell 
      proliferation and death. The effects of ethanol on cell proliferation and death 
      were examined in primary cultures of neocortical neurons derived from 16-day-old 
      rat fetuses. The cells were treated with ethanol (0 or 400 mg/dl) and examined 
      for (1) immunohistochemical identity, (2) cell cycle kinetics using a cumulative 
      bromodeoxyuridine labeling technique, (3) viable cell number via a trypan blue 
      assay, and (4) the incidence of cell death with terminal deoxy-nucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) and caspase 3 
      immunhistochemistry. After two days in culture, most (>85%) cells expressed a 
      neuron-specific antigen(s) whether or not ethanol was added to the culture 
      medium. Ethanol affected the proliferation of the cultured cells, e.g., the 
      length of the cell cycle was greater in the ethanol-treated cells than in 
      controls. The number of trypan blue-negative (viable) cells was profoundly 
      decreased by ethanol exposure. This decrease was accompanied by increases in the 
      frequencies of TUNEL- and caspase 3-positive cells and of cells exhibiting 
      nuclear condensations. Thus, ethanol decreases the number of viable cells in 
      vitro by slowing cell proliferation and increasing the incidence of cell death. 
      The expression of the death indices in untreated cultures is most consistent with 
      a single (apoptotic) pathway of cell death, rather than simultaneous apoptotic 
      and necrotic modes of death. Furthermore, it appears that ethanol initiates an 
      apoptotic death among cultured cortical neurons.
FAU - Jacobs, J S
AU  - Jacobs JS
AD  - Neuroscience Program, University of Iowa, Iowa City 52242, USA.
FAU - Miller, M W
AU  - Miller MW
LA  - eng
GR  - AA05525/AA/NIAAA NIH HHS/United States
GR  - AA06916/AA/NIAAA NIH HHS/United States
GR  - AA07568/AA/NIAAA NIH HHS/United States
GR  - AA09611/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurocytol
JT  - Journal of neurocytology
JID - 0364620
RN  - 0 (Central Nervous System Depressants)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Cell Death/drug effects/physiology
MH  - Cell Division/drug effects/physiology
MH  - Cells, Cultured
MH  - Central Nervous System Depressants/*pharmacology
MH  - Ethanol/*pharmacology
MH  - Female
MH  - Fetus
MH  - Neocortex/*cytology/*drug effects
MH  - Neurons/*cytology/*drug effects
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/04/16 10:00
MHDA- 2002/07/02 10:01
CRDT- 2002/04/16 10:00
PHST- 2002/04/16 10:00 [pubmed]
PHST- 2002/07/02 10:01 [medline]
PHST- 2002/04/16 10:00 [entrez]
AID - 10.1023/a:1015013609424 [doi]
PST - ppublish
SO  - J Neurocytol. 2001 May;30(5):391-401. doi: 10.1023/a:1015013609424.