PMID- 11952141
OWN - NLM
STAT- MEDLINE
DCOM- 20021003
LR  - 20071115
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 15
IP  - 1
DP  - 2002
TI  - Differential processing of HLA A2-restricted HIV type 1 cytotoxic T lymphocyte 
      epitopes.
PG  - 193-6
AB  - Cytotoxic T lymphocytes (CTLs) play a key role in the control of persistent viral 
      infections. Differences in the quality of this cellular immune response influence 
      the long-term outcome of such infections, but the factors that determine which 
      virus-derived peptide epitopes are targeted by CTLs remain poorly understood. 
      Here, we examine the antigen-processing requirements of three human leukocyte 
      antigen (HLA) A*0201-restricted HIV-1 CTL epitopes. Each of these three peptides 
      appears to be generated by a distinct proteolytic pathway, despite presentation 
      on the cell surface in association with the same HLA class I molecule. 
      Presentation of the commonly immunodominant SLYNTVATL (HIV-1 p17 Gag; residues 
      77-85) epitope was unaffected by inhibition of the proteasome with lactacystin, 
      but was dependent on the presence of the beta-subunit LMP7. These findings are 
      consistent with emerging data on the complexity of peptide epitope generation, 
      and suggest that differences in antigen processing might contribute to patterns 
      of CTL recognition in vivo.
FAU - Sewell, Andrew K
AU  - Sewell AK
AD  - Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, United 
      Kingdom. andy.sewell@ndm.ox.ac.uk
FAU - Booth, Bruce L Jr
AU  - Booth BL Jr
FAU - Cerundolo, Vincenzo
AU  - Cerundolo V
FAU - Phillips, Rodney E
AU  - Phillips RE
FAU - Price, David A
AU  - Price DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Multienzyme Complexes)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cysteine Endopeptidases/physiology
MH  - *Epitopes, T-Lymphocyte
MH  - HIV Reverse Transcriptase/immunology
MH  - HIV-1/*immunology
MH  - HLA-A2 Antigen/*immunology
MH  - Multienzyme Complexes/physiology
MH  - Proteasome Endopeptidase Complex
MH  - Rats
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2002/04/16 10:00
MHDA- 2002/10/04 04:00
CRDT- 2002/04/16 10:00
PHST- 2002/04/16 10:00 [pubmed]
PHST- 2002/10/04 04:00 [medline]
PHST- 2002/04/16 10:00 [entrez]
AID - 10.1089/088282402317340332 [doi]
PST - ppublish
SO  - Viral Immunol. 2002;15(1):193-6. doi: 10.1089/088282402317340332.