PMID- 11955664
OWN - NLM
STAT- MEDLINE
DCOM- 20020621
LR  - 20220311
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 40
IP  - 5
DP  - 2002 May
TI  - Local lymph node assay - validation, conduct and use in practice.
PG  - 593-8
AB  - The validation of alternative methods is a relatively new activity in toxicology. 
      The local lymph node assay (LLNA), a novel method for the identification of 
      chemicals that have the potential to cause skin sensitization, was the first test 
      to pass through the formal regulatory validation process established in the USA 
      under the auspices of ICCVAM, the Interagency Coordinating Committee on the 
      Validation of Alternative Methods. ICCVAM approved the LLNA as an alternative to 
      guinea pig tests for the identification of skin sensitisation hazards. In this 
      report, we explore the nine recommendations made by ICCVAM and discuss their 
      interpretation in relation to the new OECD Guideline 429, which describes the 
      LLNA. In particular, the value and limitations of the use of statistical 
      evaluation of data and of the inclusion of routine positive controls is examined. 
      It is concluded that the OECD Guideline as currently written embodies the 
      necessary flexibility to permit conduct of the LLNA in a manner necessary to meet 
      the varying needs of regulatory agencies and toxicologists around the world.
FAU - Basketter, D A
AU  - Basketter DA
AD  - SEAC, Unilever Colworth House, Sharnbrook, Beds MK44 1LQ, UK. 
      david.basketter@unilever.com
FAU - Evans, P
AU  - Evans P
FAU - Fielder, R J
AU  - Fielder RJ
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Radiopharmaceuticals)
SB  - IM
MH  - Animals
MH  - Dermatitis, Contact/*pathology
MH  - Drug Evaluation, Preclinical
MH  - Lymph Nodes/immunology/*pathology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Radiopharmaceuticals
MH  - Reproducibility of Results
MH  - Skin Diseases/*chemically induced
MH  - Species Specificity
RF  - 27
EDAT- 2002/04/17 10:00
MHDA- 2002/06/22 10:01
CRDT- 2002/04/17 10:00
PHST- 2002/04/17 10:00 [pubmed]
PHST- 2002/06/22 10:01 [medline]
PHST- 2002/04/17 10:00 [entrez]
AID - S0278691501001302 [pii]
AID - 10.1016/s0278-6915(01)00130-2 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2002 May;40(5):593-8. doi: 10.1016/s0278-6915(01)00130-2.