PMID- 11956562
OWN - NLM
STAT- MEDLINE
DCOM- 20021224
LR  - 20061115
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 52
IP  - 3
DP  - 2000 Jun
TI  - [Effects of bFGF and BDNF on the cells of injured adult mouse olfactory 
      epithelium in vitro].
PG  - 193-8
AB  - Olfactory epithelium (OE) is unique in the adult nervous system for it produces 
      new neurons throughout life. The cultured cells of adult mouse OE in vitro were 
      studied by histochemistry and immunocytochemistry techniques, and the results 
      showed that they were neurons as up to 50% of the bipolar cells were 
      immunopositive to NF, NSE, MAP2, OMP and tau. The effects of basic fibroblast 
      growth factor (bFGF) and brain-derived neurotrophic factor (BDNF) in OE medium 
      with different concentration serum on OE cell number and development of cell 
      processes were also observed. The results showed that the combination of bFGF and 
      BDNF added to 10% and 5% serum OE medium increased the bipolar cell number and 
      percentage, stimulated the process outgrowth of bipolar cells, and decreased the 
      numbers of giant cells and fusiform cells. The effects of the combination of bFGF 
      and BDNF in the 10% serum OE medium were more significant than those of bFGF and 
      BDNF applied alone; and the effects of neurotrophic factors in the 10% serum OE 
      medium were more significant than those observed in the 5% serum OE medium.
FAU - Zhang, X D
AU  - Zhang XD
AD  - Biotechnological Center, Suzhou University, Suzhou 215151, China. 
      zhang02@public1.sz.js.cn
FAU - Guo, Z F
AU  - Guo ZF
FAU - Liu, N
AU  - Liu N
FAU - Roisen, F J
AU  - Roisen FJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Epithelial Cells/*pathology
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Mice
MH  - Olfactory Bulb/*pathology
EDAT- 2002/04/17 10:00
MHDA- 2002/12/27 04:00
CRDT- 2002/04/17 10:00
PHST- 2002/04/17 10:00 [pubmed]
PHST- 2002/12/27 04:00 [medline]
PHST- 2002/04/17 10:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2000 Jun;52(3):193-8.