PMID- 11960348
OWN - NLM
STAT- MEDLINE
DCOM- 20020507
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 16
IP  - 4
DP  - 2002 Apr
TI  - ETV6/AML1 fusion by FISH in adult acute lymphoblastic leukemia.
PG  - 669-74
AB  - Dual-color interphase fluorescence in situ hybridization (FISH) with ETV6 and 
      AML1 probes was used for the first time on a series of 159 adult patients with 
      acute lymphoblastic leukemia (ALL), for detection of the t(12;21)(p13;q22) 
      translocation. Seven patients (4.4%) were found, with 50-100% of positive cells, 
      of whom one of two tested, proved negative for the fusion product by RT-PCR. Two 
      of them, aged 43 and 50 years, are the oldest patients so far confirmed to have 
      the translocation. Three who relapsed at 10, 11 and 24 months, suggest that 
      adults may not enjoy the good short-term prognosis reported for t(12;21)-positive 
      children. Thirty-one-negative cases had signal numbers differing from the two 
      expected for each gene. In 15 cases these results were consistent with the 
      karyotype. In nine cases with uninformative cytogenetics, the numbers were 
      consistent with those for centromeres and indicated a hidden aneuploidy. Loss of 
      ETV6 genes in two cases and AML1 amplification in three others were not suspected 
      from the cytogenetics. In conclusion, FISH proved to be reliable in defining 
      ETV6/AML1 positivity in this group of patients as well as providing valuable 
      insights into negative cases.
FAU - Jabber Al-Obaidi, M S
AU  - Jabber Al-Obaidi MS
AD  - LRF/UKCCG Karyotype Database in ALL, Department of Haematology, The Royal Free 
      and University College Medical School, London, UK.
FAU - Martineau, M
AU  - Martineau M
FAU - Bennett, C F
AU  - Bennett CF
FAU - Franklin, I M
AU  - Franklin IM
FAU - Goldstone, A H
AU  - Goldstone AH
FAU - Harewood, L
AU  - Harewood L
FAU - Jalali, G R
AU  - Jalali GR
FAU - Prentice, H G
AU  - Prentice HG
FAU - Richards, S M
AU  - Richards SM
FAU - Roberts, K
AU  - Roberts K
FAU - Harrison, C J
AU  - Harrison CJ
CN  - Medical Research Council Adult Leukaemia Working Party
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (DNA Primers)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
SB  - IM
CIN - Leukemia. 2003 Feb;17(2):476-7; author reply 477. PMID: 12592356
MH  - Adolescent
MH  - Adult
MH  - Bone Marrow/pathology
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Core Binding Factor Alpha 2 Subunit
MH  - DNA Primers/chemistry
MH  - Female
MH  - Gene Amplification
MH  - Gene Deletion
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics/metabolism
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Translocation, Genetic/*genetics
EDAT- 2002/04/18 10:00
MHDA- 2002/05/08 10:01
CRDT- 2002/04/18 10:00
PHST- 2001/07/27 00:00 [received]
PHST- 2001/12/05 00:00 [accepted]
PHST- 2002/04/18 10:00 [pubmed]
PHST- 2002/05/08 10:01 [medline]
PHST- 2002/04/18 10:00 [entrez]
AID - 10.1038/sj.leu.2402435 [doi]
PST - ppublish
SO  - Leukemia. 2002 Apr;16(4):669-74. doi: 10.1038/sj.leu.2402435.