PMID- 11962331
OWN - NLM
STAT- MEDLINE
DCOM- 20021121
LR  - 20071114
IS  - 1473-7159 (Print)
IS  - 1473-7159 (Linking)
VI  - 2
IP  - 2
DP  - 2002 Mar
TI  - FISH in cancer diagnosis and prognostication: from cause to course of disease.
PG  - 109-19
AB  - The last 20 years have witnessed an astounding evolution of cytogenetic 
      approaches to cancer diagnosis and prognostication. Molecular techniques and, in 
      particular, nonisotopically-labeled nucleic acid probes and fluorescence in situ 
      hybridization (FISH)-based techniques have replaced the costly and potentially 
      dangerous radioactive techniques used in research and the clinical detection of 
      genetic alterations in tumor cells. Fluorescent DNA probes also enabled the 
      screening for very subtle chromosomal changes. Clinical laboratories now choose 
      from a growing number of FISH-based cytogenetic tests to support physician's 
      diagnoses of the causes and the course of a disease. Depending on the specimen, 
      state-of-the-art FISH techniques allow the localization and scoring of 10-24 
      different targets and overcome previous problems associated with target 
      colocalization and detection system bandwidth. FISH-based analyses have been 
      applied very successfully to the analysis of single cells and have demonstrated 
      the existence of cell clones of different chromosomal make-up within human 
      tumors. This information provides disease-specific information to the attending 
      physician and should enable the design of patient-specific protocols for disease 
      intervention.
FAU - Weier, Heinz-Ulrich G
AU  - Weier HU
AD  - Life Sciences Division 74-157, University of California, E.O. Lawrence Berkeley 
      National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA. ugweier@lbl.gov
FAU - Greulich-Bode, Karin M
AU  - Greulich-Bode KM
FAU - Ito, Yuko
AU  - Ito Y
FAU - Lersch, Robert A
AU  - Lersch RA
FAU - Fung, Jingly
AU  - Fung J
LA  - eng
GR  - CA 80792/CA/NCI NIH HHS/United States
GR  - CA 88258/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - England
TA  - Expert Rev Mol Diagn
JT  - Expert review of molecular diagnostics
JID - 101120777
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Karyotyping
MH  - Metaphase
MH  - Neoplasms/*diagnosis/*genetics
MH  - Prognosis
RF  - 56
EDAT- 2002/04/19 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/04/19 10:00
PHST- 2002/04/19 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/04/19 10:00 [entrez]
AID - ERM020204 [pii]
AID - 10.1586/14737159.2.2.109 [doi]
PST - ppublish
SO  - Expert Rev Mol Diagn. 2002 Mar;2(2):109-19. doi: 10.1586/14737159.2.2.109.