PMID- 11962638
OWN - NLM
STAT- MEDLINE
DCOM- 20021030
LR  - 20190916
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 45
IP  - 2
DP  - 2002 Apr
TI  - RAPD isolation of a Y chromosome specific ORF in a dioecious plant, Silene 
      latifolia.
PG  - 413-20
AB  - Silene latifolia is a dioecious plant and has heteromorphic sex chromosomes: the 
      X and Y chromosomes. The Y chromosome is the largest, and its genetic control 
      seems to be most strict among dioecious plants. To identify the putative 
      sex-determination elements on the Y chromosome, random amplified polymorphic DNA 
      (RAPD) analysis was used to screen for Y chromosome specific DNA fragments, and 
      31 clones were successfully produced. Genomic Southern hybridization and FISH 
      (fluorescence in situ hybridization) analyses revealed that one of the clones, 
      #2-2, is a Y chromosome specific fragment that has a single copy on the Y 
      chromosome. Sequence tagged site (STS)-PCR analysis also succeeded in amplifying 
      one fragment in males and no fragments in females. Cloning and sequencing of the 
      #2-2 flanking region using inverse PCR revealed an open reading frame (ORF) 
      corresponding to 285 amino acids in length (ORF285), but no expression of the 
      ORF285 gene was identified. ORF285 may be a clue to the origin of dioecy.
FAU - Nakao, Shunsuke
AU  - Nakao S
AD  - Department of Biological Sciences, Graduate School of Science, University of 
      Tokyo, Hongo, Japan. nakao@biol.s.u-tokyo.ac.jp
FAU - Matsunaga, Sachihiro
AU  - Matsunaga S
FAU - Sakai, Atsushi
AU  - Sakai A
FAU - Kuroiwa, Tsuneyoshi
AU  - Kuroiwa T
FAU - Kawano, Shigeyuki
AU  - Kawano S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Plant/analysis
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Open Reading Frames/*genetics
MH  - *Random Amplified Polymorphic DNA Technique
MH  - Sequence Tagged Sites
MH  - Sex Chromosomes/genetics
MH  - Silene/*genetics
MH  - Y Chromosome/*genetics
EDAT- 2002/04/19 10:00
MHDA- 2002/11/01 04:00
CRDT- 2002/04/19 10:00
PHST- 2002/04/19 10:00 [pubmed]
PHST- 2002/11/01 04:00 [medline]
PHST- 2002/04/19 10:00 [entrez]
AID - 10.1139/g01-151 [doi]
PST - ppublish
SO  - Genome. 2002 Apr;45(2):413-20. doi: 10.1139/g01-151.