PMID- 11962737
OWN - NLM
STAT- MEDLINE
DCOM- 20021125
LR  - 20191105
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 2
IP  - 4
DP  - 2002 Mar
TI  - Effect of liposomal antigens on the priming and activation of the immune system 
      by dendritic cells.
PG  - 591-601
AB  - Dendritic cells (DCs) are recognized as the sole professional antigen-presenting 
      cells capable of priming naive T cells of the helper and cytotoxic phenotypes. 
      This property is presently exploited with success in vaccinal strategies against 
      pathogens or tumor cells that otherwise escape immune recognition, but the 
      repeated infusions of ex vivo expanded and sensitized DCs are usually required to 
      achieve protection. In this paper, we demonstrate that liposomal antigens can 
      efficiently relay and propagate the action of DCs, inducing a strong long-term 
      response against their associated antigen. Their effect is mainly achieved by 
      improving the ex vivo loading of DCs and by efficiently channeling the activation 
      stimulus into the induction of effector function. This is demonstrated by the 
      sustained immunoglobulin production as well as by the sustained 
      lymphoproliferation and the increased cytokine secretion that can be achieved 
      upon restimulation of DC-primed immune cells with limited amount of liposomal 
      antigenic material. Being well-tolerated and easily prepared, liposomal antigens 
      could therefore be expected to significantly contribute to the efficiency and to 
      a more general utilization of the highly promising but rather cumbersome DC-based 
      immunotherapies.
FAU - Shahum, Eliane
AU  - Shahum E
AD  - Groupe de Recherche en Biologie Medicale, Departement de Chimie-Biologie, 
      Universite du Quebec a Trois-Rivieres, Three Rivers, Canada.
FAU - Therien, Helene-Marie
AU  - Therien HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Antibodies)
RN  - 0 (Antigens)
RN  - 0 (Liposomes)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Antibodies/*immunology
MH  - Antigens/*administration & dosage/*immunology
MH  - Dendritic Cells/*immunology
MH  - Immunization
MH  - Interleukin-10/analysis
MH  - Interleukin-12/analysis
MH  - *Liposomes
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred AKR
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Spleen/cytology/immunology
EDAT- 2002/04/19 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/04/19 10:00
PHST- 2002/04/19 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/04/19 10:00 [entrez]
AID - S1567-5769(02)00004-8 [pii]
AID - 10.1016/s1567-5769(02)00004-8 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2002 Mar;2(4):591-601. doi: 10.1016/s1567-5769(02)00004-8.