PMID- 11964153
OWN - NLM
STAT- MEDLINE
DCOM- 20020610
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 363
IP  - Pt 3
DP  - 2002 May 1
TI  - Stimulation of erythroleukaemia cell differentiation by extracellular 
      high-mobility group-box protein 1 is independent of the receptor for advanced 
      glycation end-products.
PG  - 529-35
AB  - In several cell types the binding of extracellular high-mobility group-box 
      protein 1 (HMGB1) with the receptor for advanced glycation end-products (RAGE) 
      induces cytoskeletal reorganization and cell motility. To establish whether RAGE 
      is also involved in murine erythroleukaemia (MEL) cell differentiation stimulated 
      by HMGB1, we have demonstrated that these cells express a 51 kDa protein 
      identified as RAGE, and then we have produced stable transfectants overexpressing 
      wild-type (wt) RAGE or a dominant negative (dn) RAGE mutant lacking the 
      cytoplasmic domain to analyse the differentiation process in these cells. Several 
      experimental findings indicated that RAGE was not involved in the MEL cell 
      differentiation programme. This was also supported by the identical stimulatory 
      effect exerted by HMGB1 on both wt- or dn-RAGE transfectants. We have also 
      observed that HMGB1 binds a 65 kDa protein on the surface of MEL cells, 
      supporting the hypothesis that alternative targets of HMGB1 are expressed on the 
      MEL cell membrane and may be involved as mediators of its signalling.
FAU - Sparatore, Bianca
AU  - Sparatore B
AD  - Biochemistry Section, Department of Experimental Medicine, University of Genoa, 
      Viale Benedetto XV, 1-16132 Genoa, Italy. traspar@unige.it
FAU - Pedrazzi, Marco
AU  - Pedrazzi M
FAU - Passalacqua, Mario
AU  - Passalacqua M
FAU - Gaggero, Deborah
AU  - Gaggero D
FAU - Patrone, Mauro
AU  - Patrone M
FAU - Pontremoli, Sandro
AU  - Pontremoli S
FAU - Melloni, Edon
AU  - Melloni E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (HMGB1 Protein)
RN  - 0 (Ligands)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 0 (Receptors, Immunologic)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Membrane/metabolism
MH  - Cytoskeleton/physiology
MH  - HMGB1 Protein/*metabolism/pharmacology
MH  - Humans
MH  - Leukemia, Erythroblastic, Acute/*pathology
MH  - Ligands
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 1/antagonists & inhibitors
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors
MH  - Receptor for Advanced Glycation End Products
MH  - Receptors, Immunologic/drug effects/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
MH  - Tumor Cells, Cultured
PMC - PMC1222505
EDAT- 2002/04/20 10:00
MHDA- 2002/06/11 10:01
PMCR- 2002/11/01
CRDT- 2002/04/20 10:00
PHST- 2002/04/20 10:00 [pubmed]
PHST- 2002/06/11 10:01 [medline]
PHST- 2002/04/20 10:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 10.1042/0264-6021:3630529 [doi]
PST - ppublish
SO  - Biochem J. 2002 May 1;363(Pt 3):529-35. doi: 10.1042/0264-6021:3630529.