PMID- 11965035
OWN - NLM
STAT- MEDLINE
DCOM- 20020508
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 73
IP  - 7
DP  - 2002 Apr 15
TI  - Expression of protective genes in human renal allografts: a regulatory response 
      to injury associated with graft rejection.
PG  - 1079-85
AB  - BACKGROUND: Long-term survival of a graft requires inhibition of host immune 
      effectors, but protective responses emanating from the graft might be equally 
      important. Expression of the "protective" genes A20, heme-oxygenase-1 (HO-1), and 
      Bcl-xL in rodent allo and xenografts correlates with long-term survival. Little 
      is known of the pattern of expression of such protective genes and the 
      implication thereof in clinical transplantation. METHODS: We analyzed, by 
      quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) and 
      immunohistochemistry, expression of A20, HO-1, and Bcl-xL in 31 renal allograft 
      biopsies from patients with suspected rejection. RESULTS: A20 is not expressed in 
      nonrejecting (NR) grafts. Its expression is increased in grafts undergoing acute 
      and chronic rejection (AR and CR) but is weaker in CR. HO-1 is not expressed in 
      NR grafts; it is up-regulated in AR but not CR. Bcl-xL is detected in all 
      biopsies with decreased levels in CR. Expression of A20, HO-1, and Bcl-xL 
      localizes mainly to endothelial, smooth muscle, and infiltrating mononuclear 
      cells. CONCLUSIONS: This data demonstrate that A20 and HO-1 are up-regulated in 
      response to immune injury inferred by AR. Given the antiapoptotic and 
      antiinflammatory functions of these genes, we hypothesize that their expression 
      survives to limit graft injury by maintaining cell viability and controlling 
      inflammation. Their reduced expression in CR as compared with AR represents 
      either inadequate response to injury or a sequelae of prior injury that 
      jeopardizes further tissue response to immune attack.
FAU - Avihingsanon, Yingyos
AU  - Avihingsanon Y
AD  - Division of Immunology, Department of Medicine, Immunobiology Research Center, 
      Harvard Medical School, Boston, MA 02215, USA.
FAU - Ma, Naili
AU  - Ma N
FAU - Csizmadia, Eva
AU  - Csizmadia E
FAU - Wang, Candace
AU  - Wang C
FAU - Pavlakis, Martha
AU  - Pavlakis M
FAU - Giraldo, Mauricio
AU  - Giraldo M
FAU - Strom, Terry B
AU  - Strom TB
FAU - Soares, Miguel P
AU  - Soares MP
FAU - Ferran, Christiane
AU  - Ferran C
LA  - eng
GR  - 1 UO1 AI46134/AI/NIAID NIH HHS/United States
GR  - 5 R01 HL57791-04/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (BCL2L1 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-X Protein)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 3.4.19.12 (TNFAIP3 protein, human)
RN  - EC 3.4.19.12 (Tumor Necrosis Factor alpha-Induced Protein 3)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - DNA-Binding Proteins
MH  - Graft Rejection/*metabolism
MH  - Heme Oxygenase (Decyclizing)/analysis/*genetics
MH  - Heme Oxygenase-1
MH  - Humans
MH  - Immunohistochemistry
MH  - Intracellular Signaling Peptides and Proteins
MH  - *Kidney Transplantation
MH  - Membrane Proteins
MH  - Middle Aged
MH  - Nuclear Proteins
MH  - Proteins/analysis/*genetics
MH  - Proto-Oncogene Proteins c-bcl-2/analysis/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transplantation, Homologous
MH  - Tumor Necrosis Factor alpha-Induced Protein 3
MH  - bcl-X Protein
EDAT- 2002/04/20 10:00
MHDA- 2002/05/09 10:01
CRDT- 2002/04/20 10:00
PHST- 2002/04/20 10:00 [pubmed]
PHST- 2002/05/09 10:01 [medline]
PHST- 2002/04/20 10:00 [entrez]
AID - 10.1097/00007890-200204150-00011 [doi]
PST - ppublish
SO  - Transplantation. 2002 Apr 15;73(7):1079-85. doi: 
      10.1097/00007890-200204150-00011.