PMID- 11968052
OWN - NLM
STAT- MEDLINE
DCOM- 20021008
LR  - 20191114
IS  - 0887-8013 (Print)
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Linking)
VI  - 16
IP  - 3
DP  - 2002
TI  - Correlation of p21 gene codon 31 polymorphism and TNF-alpha gene polymorphism 
      with nasopharyngeal carcinoma.
PG  - 146-50
AB  - Background p21 (WAF1/CIP1) is a downstream protein from p53 and can arrest the 
      cell cycle at the G1/S phase in response to signal from p53. The most frequently 
      seen polymorphic site is at codon 31, where a base change from AGC to AGA causes 
      an amino acid change from serine to arginine. Tumor necrosis factor-alpha 
      (TNF-alpha) is a cytokine that is secreted from macrophages, and is related to a 
      sequence of events in the response to inflammation and cancer formation. The 
      TNF-alpha gene promoter -308 G/A polymorphism has been reported to be associated 
      with some cancers. In this study, these polymorphisms were proposed to be a 
      candidate genetic marker of nasopharyngeal carcinoma (NPC). The distribution was 
      analyzed in 47 NPC patients and a control group of 119 healthy people. The 
      association of the p21 codon 31 polymorphism with NPC was detected by polymerase 
      chain reaction (PCR) and restriction analysis by Blp I endonuclease, and 
      calculated by the chi-square test. The TNF-alpha gene promoter -308 G/A 
      polymorphism was identified by Nco I endonuclease. The distribution of the gene 
      p21 codon 31 polymorphisms showed no significant difference between the two 
      groups. The serine form of p21 codon 31 was more prominent in smokers than 
      nonsmokers among the NPC patients (P < 0.05). There was no significant difference 
      in the distribution of TNF-alpha gene promoter -308 G/A polymorphism between 
      control and cancer patients. The results indicate that the gene p21 codon 31 
      polymorphism and TNF-alpha promoter -308 polymorphism are not correlated with 
      NPC. However, the difference between smokers and nonsmokers suggests that an 
      environmental factor may be involved in association with the p21 gene in the 
      formation of NPC.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Tsai, Ming-Hsui
AU  - Tsai MH
AD  - Department of Otolaryngology, China Medical College Hospital, Taichung, Taiwan.
FAU - Chen, Wen-Chi
AU  - Chen WC
FAU - Tsai, Fuu-Jen
AU  - Tsai FJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma/*genetics
MH  - Chi-Square Distribution
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/*genetics
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nasopharyngeal Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Restriction Mapping
MH  - Tumor Necrosis Factor-alpha/*genetics
PMC - PMC6808169
EDAT- 2002/04/23 10:00
MHDA- 2002/10/09 04:00
PMCR- 2002/04/10
CRDT- 2002/04/23 10:00
PHST- 2002/04/23 10:00 [pubmed]
PHST- 2002/10/09 04:00 [medline]
PHST- 2002/04/23 10:00 [entrez]
PHST- 2002/04/10 00:00 [pmc-release]
AID - JCLA10032 [pii]
AID - 10.1002/jcla.10032 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2002;16(3):146-50. doi: 10.1002/jcla.10032.