PMID- 11969193
OWN - NLM
STAT- MEDLINE
DCOM- 20021002
LR  - 20220331
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 304
IP  - 1
DP  - 2002 May 1
TI  - Method for the simultaneous determination of retinol and beta-carotene 
      concentrations in human tissues and plasma.
PG  - 100-9
AB  - To understand differential tissue distribution of retinoids and carotenoids, as 
      it might influence biological processes in humans, we developed and demonstrated 
      a method for measuring them in selected human tissues. The method includes 
      internal standards and a secondary reference standard to eliminate the need for 
      external standard calibration and to minimize sample-handling errors. Tissues 
      were digested (saponified) in ethanolic KOH. Retinol and beta-carotene were 
      extracted with organic solvent containing internal standards. Analytes were 
      separated using isocratic liquid chromatography and quantified at 325 nm for 
      retinol and 450 nm for beta-carotene. Plasma was analyzed in a similar way but 
      without saponification. Retinal-O-ethyloxime and 
      beta-apo-12'-carotenal-O-t-butyloxime served as internal standards. Plasma, 
      breast, and fat from breast surgery patients and colon, liver, muscle, and fat 
      from colon surgery patients were analyzed. Within-day relative standard 
      deviations (RSDs) for plasma were <0.04 for beta-carotene and <0.03 for retinol, 
      between-day RSDs were <0.05 for beta-carotene and <0.04 for retinol. 
      Saponification ensured complete extraction of retinol and beta-carotene and 
      removal of triglycerides that "foul" chromatographic columns. It seems retinol 
      and beta-carotene concentrations in tissues and blood of cancer patients are the 
      same or higher than those in corresponding tissues of patients without these 
      cancers.
CI  - (c)2002 Elsevier Science (USA).
FAU - Lunetta, Jennine M
AU  - Lunetta JM
AD  - Department of Nutrition, University of California at Davis, 95616, USA.
FAU - Zulim, Rebecca A
AU  - Zulim RA
FAU - Dueker, Stephen R
AU  - Dueker SR
FAU - Lin, Yumei
AU  - Lin Y
FAU - Flaig, Vicky
AU  - Flaig V
FAU - Schneider, Philip D
AU  - Schneider PD
FAU - Wolfe, Bruce M
AU  - Wolfe BM
FAU - Clifford, Andrew J
AU  - Clifford AJ
LA  - eng
GR  - DK-43098/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 01YAE03M7J (beta Carotene)
RN  - 11103-57-4 (Vitamin A)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Chemical Analysis/methods
MH  - Breast Neoplasms/blood/chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Colonic Neoplasms/blood/chemistry
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Reference Standards
MH  - Spectrophotometry
MH  - Tissue Distribution
MH  - Vitamin A/*analysis/*blood/standards
MH  - beta Carotene/*analysis/*blood/standards
EDAT- 2002/04/24 10:00
MHDA- 2002/10/03 04:00
CRDT- 2002/04/24 10:00
PHST- 2002/04/24 10:00 [pubmed]
PHST- 2002/10/03 04:00 [medline]
PHST- 2002/04/24 10:00 [entrez]
AID - S0003269701955563 [pii]
AID - 10.1006/abio.2001.5556 [doi]
PST - ppublish
SO  - Anal Biochem. 2002 May 1;304(1):100-9. doi: 10.1006/abio.2001.5556.