PMID- 11975979
OWN - NLM
STAT- MEDLINE
DCOM- 20021009
LR  - 20191210
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 63
IP  - 5
DP  - 2002 May
TI  - HLAMatchmaker: a molecularly based algorithm for histocompatibility 
      determination. II. Verification of the algorithm and determination of the 
      relative immunogenicity of amino acid triplet-defined epitopes.
PG  - 353-63
AB  - HLAMatchmaker is a computer algorithm that assesses human leukocyte antigen (HLA) 
      compatibility at the structural level by intralocus and interlocus comparisons of 
      polymorphic amino acid triplets in antibody-accessible sequences of HLA class I 
      molecules. This program permits the identification of mismatched HLA antigens 
      that share all of their polymorphic triplets with the patient's HLA antigens and, 
      therefore, could be considered fully compatible. The validity of this algorithm 
      has been verified by analyzing the antibody specificity patterns of 127 
      well-characterized sera (panel reactive antibody [PRA] > 80%) that had been 
      screened by direct complement-dependent and/or anti-human globulin augmented 
      lymphocytotoxicity testing with large HLA-typed cell panels. A 2 x 2 table-based 
      Chi-square analysis program was applied to determine positive and negative 
      correlations between serum reactivity and the presence HLA triplets assigned from 
      the HLA types in the cell panel. The results indicate that high PRA patients do 
      not produce antibodies to shared triplets on mismatched HLA antigens. Moreover, 
      this serum analysis has permitted the identification of triplets with different 
      degrees of immunogenicity as indicated by the frequencies of positive and 
      negative correlations of serum reactivity with the HLA-typed cell panel. 
      Mismatching for triplets with low immunogenicity provides further opportunities 
      for identifying donors with acceptable HLA mismatches for highly sensitized 
      patients.
FAU - Duquesnoy, Rene J
AU  - Duquesnoy RJ
AD  - Department of Pathology, Division of Transplantation Pathology and the Thomas E. 
      Starzl Transplantation Institute, University of Pittsburgh Medical Center, 
      Pennsylvania 15261, USA. duquesnoyr@msx.upmc.edu
FAU - Marrari, Marilyn
AU  - Marrari M
LA  - eng
GR  - DK-52803/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Validation Study
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Amino Acids)
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - *Algorithms
MH  - Amino Acid Sequence
MH  - Amino Acids/analysis
MH  - Antibodies/immunology
MH  - Antibody Specificity
MH  - Chi-Square Distribution
MH  - Computational Biology/methods
MH  - Decision Making, Computer-Assisted
MH  - Epitopes/*chemistry/*immunology
MH  - HLA Antigens/chemistry/*genetics/*immunology
MH  - HLA-A Antigens/classification/genetics
MH  - HLA-B Antigens/classification/genetics
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Polymorphism, Genetic
EDAT- 2002/04/27 10:00
MHDA- 2002/10/10 04:00
CRDT- 2002/04/27 10:00
PHST- 2002/04/27 10:00 [pubmed]
PHST- 2002/10/10 04:00 [medline]
PHST- 2002/04/27 10:00 [entrez]
AID - S0198885902003816 [pii]
AID - 10.1016/s0198-8859(02)00381-6 [doi]
PST - ppublish
SO  - Hum Immunol. 2002 May;63(5):353-63. doi: 10.1016/s0198-8859(02)00381-6.